Methods in Molecular Biology • 16 Enzymes of Molecular Biology

Index

A

AatI, 130, see also Restriction enzymes
AatlI, 130, see also Restriction enzymes
AccI, 130, see also Restriction enzymes
AcclI, 130, see also Restriction enzymes
AcclII, 131, see also Restriction enzymes
Actinomycin D, 81, see also Reverse
transcriptase
AcyI, 131, see also Restriction enzymes
AfiI, 131, see also Restriction enzymes
AfilI, 131, see also Restriction enzymes
AfillI, 131, see also Restriction enzymes
AhaI, 131, see also Restriction enzymes
AhalI, 131, see also Restriction enzymes
AhalII, 131, see also Restriction enzymes
Alkaline phosphatase, 331-356
3- [2'-spiroadamantane]-4-methoxy-
4- [3-phosphoryloxyl]-phenyl-
1,2-dioxetane, see AMPPD
5-bromo-4-chloro-3-indoly-
phosphate, see BCIP
AMPPD, 332
bacterial alkaline phosphatase, 332
molecular mass, 332
BCIP, 332
calf intestinal alkaline phosphatase, 332
cations, 334
double-stranded DNA, 333
enzyme assay, 335
enzyme reaction, 333
experimental procedures, 335
inhibitors, 334
molecular mass, 333
pH, 334

protocol, 336

reaction conditions, 336

RNA, 333

single, 333

storage and stability, 335

substrate, 333

sulfhydryl reagents, 335

temperature, 334

unit definition, 335

calf intestinal mucosa, 331

CAP, 331

CIAP, 331

the enzyme, 332

Escherichia coli, 331

NBT, 332

nitroblue tetrazolium chloride, see NBT

AluI, 131, see also Restriction enzymes

Alw21I, 131, see also Restriction

enzymes

Alw26I, 131, see also Restriction

enzymes

Alw44I, 131, see also Restriction

enzymes

AlwI, 131, see also Restriction enzymes

AlwNI, 131, see also Restriction enzymes

Aminoacyl L-proline hydrolase, 324

Aminopeptidases, 319-329

5-oxoprolyl-peptidase, 321

aminopeptidase, 320

aminopeptidase M, 322-324

cyclization of N-terminal glytamine,

319

enzyme data, 321

hydrolysis of asparagine and

glutamine, 320

357