Methods in Molecular Biology • 16 Enzymes of Molecular Biology

358 Index

iminopeptidase, 319
L-pyroglutamyl peptide hydrolase, 321
loss of serine and threonine, 320
loss of tryptophan, 320
metalloprotease, 320
peptidase D, 324
prolidase, 319, 324, 325
Pseudomonas fluoresens, 320
pyroglutamase, 321
pyroglutamate aminopeptidase, 321
pyroglutamyl peptidase, 321
pyrrolidonyl peptidase, 321
Aminopeptidase M, 322-324
activation, 324
amino acid arylamidase, 322
aminoacyl peptidase hydrolase, 322
assay, 323
inhibition, 324
microsomal alanyl aminopeptidase, 322
molecular mass, 323
pH optimum, 323
specificity, 323
stability, 323
sulfhydryl reagents, 324
AocI, 132, see also Restriction enzymes
AoclI, 132, see also Restriction enzymes
AosI, 132, see also Restriction enzymes
AoslI, 132, see also Restriction enzymes
ApaI, 132, see also Restriction enzymes
ApaLI, 132, see also Restriction enzymes
ApyI, 132, see also Restriction enzymes
AquI, 132, see also Restriction enzymes
AseI, 133, see also Restriction enzymes
AsnI, 133, see also Restriction enzymes
Asp700, 133, see also Restriction enzymes
Asp718, 133, see also Restriction
enzymes
AspHI, 133, see also Restriction enzymes
AspI, 133, see also Restriction enzymes
Assay, see under each enzyme
AsuI, 133, see also Restriction enzymes
AsulI, 133, see also Restriction enzymes
ATEE, 280, 307
ATP: 5' dephosphopolynucleotide 5'
phosphatase, see Polynucleotide
kinase

AvaI, 133, see also Restriction enzymes

AvalI, 133, see also Restriction enzymes

AvalII, 134, see also Restriction enzymes

AvilI, 134, see also Restriction enzymes

AvrI, 134, see also Restriction enzymes

AvrlI, 134, see also Restriction enzymes

AxyI, 134, see also Restriction enzymes

B

Bacillus stearothermophilus, 27

uses of Taq polymerase, 28

Bali, 134, see also Restriction enzymes

BAL 31 nucleases, 231-251

Alteromonas, 231

Alteromonas espejiana (ATCC

29659), 231

assay single stranded DNA, 240-242

Ca 2÷, 235

denaturing agents, 236

duplex exonuclease, 238

duplex exonuclease activity, 240,

242-247

base composition, 246, 247

nuclease concentration, 245,246

experimental procedures, 239, 247

fast F, 231

guanidinium hydrochloride, 237

inhibitors, 236

ionic strength, 236

ions, 235

K m, 240

lesions in nonsupercoiled closed

DNA, 247, 248

Mg 2+, 235

manipulation of nucleic acids, 234

Pseudomonas, 231

pH, 235

physical features, 232

proteolysis, 238

reactions catalyzed, 232-234

duplex DNA, 232

duplex RNA, 232

single-stranded tails, 232

slow S, 231

temperature, 235