Index 369
Substrate, see each enzyme
Sulfhydryl reagents, see each enzyme
T
T4 RNA ligase, 223, 225
cations, 224
cofactors, 224
enzyme assay, 224
enzymic reaction, 224
experimental procedures, 225
reaction conditions, 225
storage and stability, 225
uses of, 225
TaqI, 159, see also Restriction enzymes
TaqI Mtase, 207, see also DNA
methyltransferase
Taq polymerase, 31-58
application of Taq polymerase in
PCR, 38
allele specificity, 46
amount of enzyme, 41
applications, 44
avidin, 39, 40
buffers, 38
clinical diagnosis, 45
dimethylsulfoxide (DMSO), 33
DMF, 33
Duchenne Muscle Dystrophy
(DMD), 45
gelatine, 38
hematin, 41
HPLC techniques, 39, 40
hypervariable, 45
inhibitors, 33
melting temperatures, 39, 40
Mg 2÷, 38
minisatellite, 45
mRNA amplification, 47
oligodeoxynucleotide, 39, 40
PAGE with 7M urea, 39, 40
point mutations, 40
primer, 39
primer DNA duplex, 39, 40
produc t identification, 45
proteinase K, 41
purification, 39, 40
reliablity, 44
SDS, 33
sequencing, 49-51
shadow casting, 39, 40
single locus analysis, 45
site directed mutagenesis, 50
standard PCR protocol, 43
streptavidin, 39, 40
template, 41
thermocycler, 42
urea, 33
enzymology, 31-33
fundamentals of the PCR, 34
Klenow, 34
m13 system, 32
polymerase chain reaction (PCR), 34
template, 35
thermophile, 31
thermostable, 32
Thermus aquaticus, 31
YT-1, 31
Temperature, see each enzyme
Terminal deoxyribonucleotidyl trans-
ferase, 95-106
applications, 96
function, 95
enzyme structure, 96
experimental procedures, 100
annealing, 103
double-stranded, 102
linearized vector, 103
tailing buffer, 101
molecular mass, 96
reaction, 95
reaction conditions, 97
buffer, 97
ion requirements, 98, 99
substrate/template, 98
Th. flavus, 27
Th. litoralis, 27
Thai, 159, see also Restriction enzymes
Thermolysin, 291-293
assay, 292
Bacillus thermoproteolyticus, 291
inhibitors, 292