Dairy Chemistry And Biochemistry

344 DAIRY CHEMISTRY AND BIOCHEMISTRY

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Figure 8.2. Schematic representation of a competitive enzyme-linked immunosorbent assay
using (a) immobilized antigen or (b) immobilized antibody.

Examples of the use of ELISA in dairy analyses include:

quantifying denaturation of fl-lactoglobulin in milk products (native and

denatured fl-lg react differently with antibodies);

detection and quantitation of adulteration of milk from one species with

that from other species, e.g. sheep's milk by bovine milk;

authentication of cheese, e.g. sheep's milk cheese;

detection and quantitation of bacterial enzymes in milk, e.g. from psy-

chrotrophs;