Antigen-Antibody Enhancement Immunohematology Review 458
REAGENT EXPLANATION
Albumin
Low ionic strength solution (LISS)
Polythyleneglycol (PEG)
Enzymes
22% bovine serum albumin. Reduces net neg charge of RBCs, allowing them to come closer together.
Lowers ionic strength of suspending medium, allowing ags & abs to move closer together more
rapidly. Reduces incubation time for IAT.
Increases ab uptake. Used for detection & ID of weak IgG abs.
Ficin & papain most commonly used. Reduce RBC surface charge by cleaving sialic acid molecules.
M, N, S, Fya, Fybantigens destroyed.
