Semen Analysis
Urinalysis and Body
Fluids Review 521
FERTILITY TESTING POST-VASECTOMY
Specimen collection
Liquefaction
Volume
Motility
Cell count
Morphology
pH
Condom can be used. Time & temp not critical.
Test monthly beginning 2 months after vasec-
tomy. Continue until 2 consecutive specimens
are without sperm.
Don’t analyze until specimen is liquified.
NA.
NA.
Examine undiluted & following centrifugation.
Even 1 sperm is significant.
NA.
Collect in sterile container, without condom, after
3-day abstinence. Keep at RT. Deliver to lab within
1 hr of collection.
Don’t analyze until specimen is liquified. (Normally
within 30 min of collection.)
Normal: 2–5 mL
Observe within 3 hr of collection. 50%–60% of sperm
should show at least fair motility.
Dilute & count in Neubauer hemacytometer.
Normal: >20 million per mL.
Stain & examine at least 200 cells. Normal: oval head
(3 × 5 μm) with long tapering tail. Abnormalities:
double heads, giant heads, amorphous heads, pinheads,
tapering heads, constricted heads, double tails, coiled
tails, large numbers of spermatids (immature forms).
Normal = <30% abnormal forms.
7.3–8.3