Basic MDx Terminology
Molecular Diagnostics
(MDx) Review 541
Molecular diagnostics
Extraction
RNase
DNase
Target
Hybridization
Hybrid
Duplex
Stringency
Analysis of DNA & RNA; nucleic acid testing (NAT).Isolation of DNA/RNA from other cellular components.Enzyme that degrades RNA. Ubiquitous in environment.Enzyme that degrades DNA.Specific section of DNA under investigation.Pairing of complementary strands of nucleic acid, 1 from sample & 1 a reagent. Resulting hybrids can be
DNA:DNA, DNA:RNA, or RNA:RNA.Product of hybridization.Hybrid.Stability of bonding during hybridization. Based on degree of match & base composition. Influenced
by temp, pH, & salt concentration. ↑temp, ↓salt ensure only most perfectly matched strands will
remain paired.continued...