Quick Review Cards for Medical Laboratory Science

Steps in PCR

Molecular Diagnostics

(MDx) Review 555

STEP TEMP/TIME* EXPLANATION

1. Denaturation (melting) dsDNA separated into 2 strands by heat. Each strand serves as template for
   synthesis of new strand.

90 °–96°C/

20–60 sec.

3 ¢

3 ¢

Region to be DNA

amplified

5 ¢

5 ¢

Primers

(From Buckingham L. Molecular Diagnostics: Fundamentals, Methods, and

Clinical Applications, 2nd ed. Philadelphia: FA Davis; 2012:133.)

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