Quick Review Cards for Medical Laboratory Science

Steps in PCRcontinued

Molecular Diagnostics

(MDx) Review 556

STEP TEMP/TIME* EXPLANATION

2. Annealing (hybridization) Primers attach to both template strands by binding with complementary
   bases on 3’ side of region to be amplified.

50 °–70°C/

20–90 sec.

3 ¢

3 ¢

DNA

5 ¢

5 ¢

Primer Primer

Region of interest

(From Buckingham L. Molecular Diagnostics: Fundamentals, Methods, and

Clinical Applications, 2nd ed. Philadelphia: FA Davis; 2012:134.)

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