Statistical Testing
The ratio of plaquing efficiency following mutation of each ICAP relative to SAG1 mutation was compared to a mean of 1 using one-
tailed t tests, and the resultingpvalues were corrected using the Benjamini-Hochberg method. Based on the small sample size, mu-
tants with a correctedpvalue lower than 0.1 were considered significant. ICAP mutant invasion efficiency was analyzed similarly us-
ing PLP1 as the control. The effects of inducing CLAMP knockdown on invasion, egress, and MIC2 secretion were analyzed in Prism
(GraphPad) using two-tailed t tests with apvalue of 0.05 or lower indicating significance. Where appropriate, statistical parameters
including the exact value ofn, the definition of center, dispersion, and precision measures and statistical significance are reported in
the figures and corresponding legends.
DATA AND SOFTWARE AVAILABILITY
Software
Guide selection and screen analysis were performed using custom software that will be provided upon request. Statistical analyses
were performed in R (http://www.R-project.org) using built-in packages unless otherwise indicated.
Data Resources
All data from the CRISPR screens is available inTable S3, and will be integrated into an upcoming release of ToxoDB (http://ToxoDB.
org).
e7 Cell 167 , 1423–1435.e1–e7, September 8, 2016