Supplemental Figures
Figure S1. RAB4 Is Not Essential forT. gondiiGrowth in Human Fibroblasts, Related toFigure 3
(A) Diagram of theRAB4locus illustrating the knockout strategy and position of the primers used for validation. Specific primers were used to amplify the 5^0
(PCR1) and 3^0 (PCR2) junctions, and a segment of the coding sequence (PCR3).
(B) PCR assay for integration of the pyrimethamine-resistance cassette into theRAB4locus and deletion of theRAB4coding sequence.
(C) RT-PCR probing for expression of RAB4 or the control ACT1 on cDNA samples prepared with or without reverse transcriptase (RT).
(D) Plaque assay demonstrating normal growth of theRAB4knockout in human fibroblasts.