Cell - 8 September 2016

A

B

C

0

5000

10,000

15,000

20,000 MT1: CT26

0

2000

4000

6000

8000

10,000 MT1: B16

0

2000

4000

6000 MT2: CT26

0

500

105

105

104

104

103

102 103

0

0100 102 103104 5

1000

1500

2000

2500 MT2: B16

TIL.DN TIL.SP TIL.DP

0

20,000

40,000

60,000

80,000

100,000

0

10

20

30

40

Zinpyr-1 MFI

Free Zn in CD8+ TILs

• 
  

WT: TIL CD8 MT–/–: TIL CD8

10.6 29.2

WT MT–/–

GrzmB

EffMem

DP (Tim3+PD1+)

SP (Tim3–PD1+)

DN (Tim3–PD1–)

WT

MT–/–

RE

RE

Granzyme B

% Granzyme B

+ CD8

+ TILs

CD8

***NS

Figure S2. Metallothionein Expression, Zinc Dysregulation, and Effector Function in CD8+TILs, Related toFigure 2
(A) Expression of MT1 and MT2 as determined by qPCR in sorted CD8+TILs isolated from mice bearing CT26 colon carcinoma and B16 melanoma tumors.
(B) WT andMT
/
TILs were stained with Zinpyr-1 for measuring free Zn followed by cell surface staining and analyzed by flow cytometry. C) Granzyme
expression in WT andMT
/
CD8+TILs.