Cell - 8 September 2016

Figure S3. Overlay of Naive and In Vitro Activated CD8+T Cell Transcriptomes on PC1 and PC2, Related toFigure 3
Naive CD8+CD62LhiCD44lowwere isolated from C57BL/6 mice by cell sorting and stimulated in vitro with plate bound anti-CD3/anti-CD28. On day 3, cells were
harvested and following RNA sequencing where overlaid onto the PCA plot generated from the DN, SP and DP subpopulations of WT andMT
/
TILs (STAR
Methods).