Stem Cell Processing (Stem Cells in Clinical Applications)

223

H
Harvesting techniques
Coleman technique , 207–211
dry technique , 206
laser-assisted liposuction , 206
liposuction vs. biopsy/resection , 204–205
power-assisted liposuction , 206
super-wet technique , 206
ultrasound-assisted liposuction , 206
water-assisted liposuction , 206
wet/tumescent technique , 206
Heart disease , 90
Hematopoietic stem cells (HSCs) , 2
Hematopoietic stem cell transplantation
(HSCT) , 164–166
Hybrids , 9
Hypoxia-inducible factor 1 (HIF-1)
heterodimer , 214

I
Idiopathic pulmonary fi brosis (IPF) , 52
Illouz method , 206
Induced pluripotent stem cells (iPSCs)
advantages , 79
clinical conditions
adenovirus vectors , 81
animal composition-free culture
systems , 82 , 83
KSR and mTeSR1 , 83
lentiviral vectors , 81
less invasive techniques , 82
mRNAs , 82
peripheral blood cells , 82
transgene system , 81
viral-free vectors , 82
xeno-free media , 83
cloning capacity , 79
c-Myc, Sox2, Klf4, and Oct4 , 79
disease modeling , 84–86
doxycycline , 79
drug screening , 85 , 87
factors , 78
GMP suite , 84
history , 77 , 78
nonviral methods , 79
obstacle, reprogramming in , 80
oncogenic transcription , 79
potential genotoxic effects , 79
quality control testing , 84
regenerative medicine , 79
cardiac differentiation , 89 , 90
diabetic mellitus , 90 , 91

EB formation , 88

generation protocols , 87

liver disease , 90 , 91

neural differentiation , 88

SMAD signaling pathway , 88

somatic cells, reprogramming , 87

TGF-β receptors , 88

retroviruses/lentiviruses , 79

RNA molecules , 80 , 81

RPE sheets , 91

transposon systems , 80

Inner cell mass (ICM) , 99

International Medical Device Regulators

Forum (IMDRF) , 23

International Society for Cellular Therapy

(ISCT) , 132

Inverted terminal repeats (ITRs) , 80

iPSCs. See Induced pluripotent stem cells

(iPSCs)

K

Kidney diseases , 52 , 53

L

Liver disease , 52 , 53 , 90 , 91

Lung disease , 52 , 53

M

Mesenchymal stem cells (MSCs)

advantages , 3

ageing

adult MSCs , 100

causes changes , 100

cell surface characterisation , 103

and differentiation ,

102 , 103

embryonic stem cells , 99

HLA-2 markers , 100

inner cell mass , 99

osteoarthritis , 101

and proliferation , 101 , 102

regenerative medicine , 100

allogeneic MSCs , 109

AlloStem , 46

autoimmune diseases , 51 , 52

CardioRel ® , 44

cardiovascular diseases , 51

CARTISTEM ® , 44

chronic infl ammatory , 51 , 52

diabetes mellitus , 53

Index