Subject Index 463
G-tetrad
G-quartet 53
guanine
base structure 14
nucleoside & nucleotide 15–16
guanine, 7-alkyl
apurinic site formation 324
Gulland, M.
nucleic acid research 4
hairpin loops 61, 62
free energy increments 63
hairpins
DNA 40
guanine-hairpin 375
kissing hairpin loops 257, 258
polyamide ligands 365
ribozyme 286
RNA 61–63, 198
H-DNA 52
Heck reaction 95, 302
helix lengthening
intercalation 345, 350
helix unwinding
duplex lengthening 349
helix-coil transitions 64, 431
helper cells 192
heterochromatin 216, 217
higher-order DNA structure 68
histone 217, 391, 408
acetylation 230
HIV 124
Hoechst 33258
DNA binding 366–368
Holley, R.
tRNA structure 7
Holliday junction 43
homologous recombination 215, 333
Hoogsteen duplex 55
human genome 221
hybridisation 172, 188
base composition 179
detection of DNA sequences 178
detection of NA sequences 178–188
temperature dependence 179
hydration
DNA 346–347
hydrogen bond
acceptors 17, 384–387
donors 17, 384–387
hydrogen bonding
G:U recognition in RNA
minor groove 255
G-tetrad 375
lexitropsin in DNA minor groove 365
neighbouring molecule contacts
crystal structures 439
structural aspects 17, 61, 67
water molecules in DNA
grooves 386
Watson-Crick for thymine
photodimer 318
8-hydroxyguanine
hydroxyl radical product 322
lyase repair 328
5-hydroxymethyl-2-deoxyuridine
-irradiation product 322
hypochromicity 19, 64, 428ifosphamide
anti-cancer agent 128
infrared spectroscopy 432–433
Fourier transform, FTIR
nucleic acid crystals 432
inosine, 2-deoxy 191
inosine 5-monophosphate
biosynthesis 118
insertions
oligonucleotide 189
integrase
RNA viruses 220
interactions
protein-nucleic acids
kinetics & thermodynamics 398–404
RNA-protein 416–421
intercalation 347–360
ethidium
dependence on DNA
conformation 440
helix lengthening 345, 350
phosphate spacing increase 345
interference RNA, RNAi 197
inter-phosphate spacing
increase on intercalation 345
intron
gene structure 210, 264
group I 266, 267
group II 266, 268, 281
group II reverse-splice 268, 269
self-splicing 266
5-iodo-2-deoxyuridine
synthesis 93
ionizing radiation and
nucleic acids 322–323
isotopic labelling
bases(^15) N 436
Jeffreys, A.
DNA fingerprinting 181
Khorana, G.
gene synthesis 9, 147
trinucleotide synthesis 8
Klenow fragment
DNA polymerase 168
lac operon
E. coli transcription 226
lacrepressor
E. colitranscription regulation 226
lagging strand
DNA strand polarity 232
leading strand
DNA strand polarity 232
lexitropsins
DNA binding 365
ligand binding
adriamycin 350
anthracycline antibiotics 350
anthraquinones 360
change in heat capacity 352
co-operativity 372
daunomycin 350–352
energetics; Gibbs relationship 352, 364
enthalpic & entropic
components 352, 364, 369
favourable hydrophobic
transfer 349, 354
fluoresence analysis 429
free energy parsing 364
hydrophobic interactions 352
intercalation vsgroove
FRET analysis 430
intercalation vsminor groove 370
isothermal titration calorimetry 444
kinetic & equilibrium constants 360
lexitropsins 365
thermodynamic analysis 354, 368
triplex selective ligands 374, 375
ligand interactions
co-operativity of 372
4 ,6-diamidino-2-phenylindole,
DAPI 371
DNA groove binding 342
DNA intercalation 342, 345
DNA outside-edge binding 342
dsDNA 342
minor groove 361–369
quadruplex DNA 375–378
salt effects 344
spermine, spermidine,
outside-edge 345
triplex DNA 372–374
light scattering 441–442
dynamic light scattering
hydrodynamic size of DNA 442
linear dichroism
study of DNA 432
transition moment 432
locked nucleic acids, LNA
backbone modification 163
L-RNA
therapeutic oligonucleotide 202
major groove
agents 310
-sheet binding
integration host factor, IHF 394, 395
met repressor 394, 395