4 Notes
- Never add glucose to the medium because glucose inhibits
bioluminescence ofVibrio harveyi. - The chance of identifying true positive compounds can be
improved if multiple scoring functions are combined. It
means that (a) each of the individual scoring functions has
relatively high performance and (b) the individual scoring
functions are distinctive [34]. - Make sure that they form at least one hydrogen bond and
hydrophobic interaction with the LuxP protein. - DPD was synthesized following literature procedures before
biological evaluation [35].
Acknowledgment
This project was funded by the National Program on Key Basic
Research Project of China (No. 2013CB734002), the National
Natural Science Foundation of China (No. 81370085 and
81001362), the Qilu Professorship at Shandong University, and
the Taishan Scholarship Program at Shandong Province.References
- Atkinson S, Williams P (2009) Quorum sens-
ing and social networking in the microbial
world. J R Soc Interface 6:959–978 - Miller MB, Bassler BL (2001) Quorum sensing
in bacteria. Annu Rev Microbiol 55:165–199 - Smith JA, Wang J, Nguyen-Mau SM, Lee V,
Sintim HO (2009) Biological screening of a
diverse set of AI-2 analogues inVibrio harveyi
suggests that receptors which are involved in
synergistic agonism of AI-2 and analogues are
promiscuous. Chem Commun 7:7033–7035 - De Kievit TR, Iglewski BH (2000) Bacterial
quorum sensing in pathogenic relationships.
Infect Immun 68:4839–4849 - Dong YH, Wang LH, JL X, Zhang HB, Zhang
XF, Zhang LH (2001) Quenching quorum-
sensing-dependent bacterial infection by an
N-acyl homoserine lactonase. Nature
411:813–817 - Dunlap PV (1999) Quorum regulation of
luminescence inVibrio fischeri. J Mol Microbiol
Biotechnol 1:5–12 - Dunny GM, Brown BL, Clewell DB (1978)
Induced cell aggregation and mating in Strep-
tococcus faecalis: evidence for a bacterial sex
pheromone. Proc Natl Acad Sci U S A
75:3479–3483- Hall-Stoodley L, Stoodley P (2005) Biofilm
formation and dispersal and the transmission
of human pathogens. Trends Microbiol
13:7–10 - Matsusaki H, Chinachoti N, Sonomoto K,
Ishizaki A (1998) Purification, identification,
and effective production of a peptide antibiotic
produced by Lactococcus lactis IO-1 (JCM
7638). Ann N Y Acad Sci 864:422–427 - Jiang T, Li M (2013) Quorum sensing inhibi-
tors: a patent review. Expert Opin Ther Pat
23:867–894 - Bassler BL, Wright M, Silverman MR (1994)
Multiple signalling systems controlling expres-
sion of luminescence in Vibrio harveyi:
sequence and function of genes encoding a
second sensory pathway. Mol Microbiol
13:273–286 - Camilli A, Bassler BL (2006) Bacterial small-
molecule signaling pathways. Science
311:1113–1116 - Eberhard A, Burlingame AL, Eberhard C, Ken-
yon GL, Nealson KH, Oppenheimer NJ
In silicoIdentification of AI-2 Inhibitors 361