Quorum Sensing

LuxN............................................................74, 355

LuxO.................................................262, 354, 355

LuxP......................... 74,354, 355, 357, 359, 361

LuxQ.................................................354, 355, 359

luxR...............4–7, 17, 18, 21, 36, 145–157, 205,

207, 262, 266, 318, 319, 354, 355

luxS ................................... 74, 78, 81,84–87, 355

LuxU.........................................................354, 355

MvfR (PqsR)..................................................... 228

OryR .................................................................. 156

PauR................................................................... 156

PluR................................................................... 156

pqsA...........................................27, 28, 30, 32, 33

pqsH..............................................................26, 28

PqsL..................................................................... 26

PsoR................................................................... 156

rhlI ........................... 25,162, 163, 165, 166, 203

rhlR .............................25, 26,203, 213, 228, 276

rpfC.......................................................98, 99, 250

rpfF......................................................97,193, 250

RpfG.................................................................. 243

RpfR................................................................... 193

ScbR................................................................... 117

SdiA...........................................................146, 147

traI ..........................................................................4

traR ........................ 4, 5,146, 150, 151, 156, 306

R

Radiolabel assay.............................. 35, 36, 38,40–43, 45
Regulon......................................146, 152–154, 178, 179
Reporter strain.........................................4, 8, 16, 17, 21,
33, 36, 38, 91–94, 118, 125, 247, 290, 291
Reverse-phase high performance liquid chromatography
(RP-HPLC)..............38, 41, 43, 98,195, 199
Reverse transcriptase polymerase chain reaction
(RT-PCR)..................................181, 186, 199
Rhamnolipid.....................................................25, 26, 204
RNA
electrophoresis.........................................................183
isolation.................................................185, 189, 284
sequencing (RNAseq).........147, 152, 177–183, 185,
187–189, 191
RNAIII................................................................... 90, 363
RNAseq library.............................................178, 186–188

S

S-adenosylmethionine (SAM)..................................36, 37
Screening.............3–7, 9, 10, 12–21, 49, 133, 134, 152,
154, 157, 287–294, 299, 344, 355, 361, 363
Secondary antibodies
for fluorescence staining .........................................216
for immunoblot analysis .........................................216
Siderophore ..................................................................... 74
Single chain antibodies (scAbs)..........................326, 330,
348, 349, 351

Sodium dodecyl sulfate-polyacrylamide gel

electrophoresis (SDS-PAGE).................77–81,

86, 137, 149, 153, 194, 195, 197, 219, 221,

314, 317

Solid phase extraction (SPE).................................. 64, 66,

108, 111, 113, 115

Staphylococcal quorum sensing inhibitors.........363–370

Strains used for bioassays.................................................. 6

Structure-based virtual screening.................................361

Supernatant.......................................... 15,27–29, 31–33,

38, 41, 43, 52–54, 58, 62, 64–66, 71, 79, 81,

83, 85, 87, 91–94, 98, 100–104, 122, 127,

152, 153, 182, 187, 197, 200, 208, 219, 221,

232, 249, 292–294, 301–303, 307, 317, 336,

338, 340, 343, 344, 348, 350, 364–366

T

Thin layer chromatography (TLC)...................... 6, 8, 11,

14, 18–20, 27–33, 61

Time-of-flight mass spectrometry (TOF-MS)

analysis......................................................66, 98

Time-resolved fluorescence lifetime............135, 139–141

Transcriptional

fusion..............................................................152–155

regulator ...........................................4, 25, 26, 36, 90,

161, 193, 203, 213, 290, 354

Transcriptomes..............................................................178

Transepithelial electrical resistance (TER)..........216–217

Transposon ..........................................149, 154–157, 290

Two-dimensional (2-D) gel electrophoresis................194

Type III secretion ........................................................... 74

U

Ultra-high performance liquid chromatographic

(UHPLC)....................................61–67, 69–71

Ultra performance liquid chromatographic

(UPLC)......................... 66,97–102, 104, 105

V

Violacein...........................................................6, 7, 15, 18

Virulence...............................................25, 26, 89, 90, 97,

108, 131, 145, 161, 194, 227–229, 243–251,

275, 288, 291, 297, 311, 326, 353, 364

Virulence factor.................................................25, 26, 89,

90, 108, 131, 145, 227, 228, 275, 288, 291,

297, 326

W

Whole-cell biosensors...................................................298

X

Xanthine oxidase ........................164, 165, 170, 171, 175

QUORUMSENSING

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