N
N-acyl-homoserine (HS) .................................... 4,61–67,
69–71, 203–211, 297, 307, 311
Next-generation sequencing (NGS)..........177, 180, 191
Niclosamide...................................................................289
Not-so-random (NSR) primers..........178, 180, 186, 190
Nuclear magnetic resonance (NMR).................... 27, 118
O
o ̃-hemolysin (o ̃-toxin)..........................................369, 370
Ordinary differential equations (ODE)......255, 257–264
P
Paracellular
flow .................................................................217–216
permeability.............................................................218
Partial differential equations (PDE)...................264–269
p-coumaroyl homoserine lactone (p-C-HSL) .........38, 42
Persistence.....................................................................228
Phage display..............................................326, 329–332,
338, 341, 343
Plant
associated bacterial (PAB) LuxR solos.......... 147, 151
colonization...................................244, 245, 248–251
phytopathogen........................................................251
Plate-based bioassay..................................................15–18
PluR/PpyS system ........................................................156
Polymerase chain reaction (PCR)........................ 65,108,
149, 152, 154, 181, 184, 185, 187, 189, 300,
313–316, 318–320, 326, 331, 338–340, 342
Potassium iodide quenching...............................135, 136
Primary antibodies
for fluorescence staining .........................................215
for immunoblot analysis .........................................216
Prodigiosins..........................................................118, 123
Protein
expression.......................................................137, 349
preparation
extracellular....................................................... 196
intracellular........................................................ 197
membrane.......................................................... 197
purification ..........................................................79, 86
Proteobacteria................................... 35, 38, 43,145, 146
Proteomics.....................................................................200
Pyocyanin...........................................................26, 27, 30,
32, 33, 108, 291–294
Q
Quinolones
2-alkyl-4-quinolones (AQ).........................25–30, 32,
33, 49, 199
detection.................................................25–30, 32, 33
extraction................................................................... 27
2-heptyl-3-hydroxy-4-quinolone or Pseudomonas
quinolone signal (PQS) ...............26,108, 288
2-heptyl-4-quinolone N-oxide (HQNO).........26, 50,
52–54, 56
2-heptyl-4-quinolone or 2-heptyl-4-hydroxyquinoline
(HHQ)....................................................26, 27,
30–33, 50, 52–55, 57, 108–114
4-hydroxy-2-alkylquinolines (HAQ) .................49–58
2-nonyl-4-hydroxyquinoline or 2-nonyl-4-quinolone
(NHQ)........................................................... 26
quantification............................................................. 49
Quorum quencing (QQ)
bacteria............................................................297–302
enzymes.................................................298, 303–305,
312, 314, 315, 317, 320–322
acylase.......................................................298, 306
directed evolution.....................................311–323
lactonase
AiiA...........................................................303, 305
GKL .........................................................312, 314,
315, 317, 320–322
MomL.......................................................298, 304
Quorum sensing (QS)......................................4–7, 9, 17,
18, 21, 25–28, 30, 32, 33, 36, 38, 41, 74, 78,
81, 84–87, 90, 91, 94, 95, 97–99, 117,
131–142, 145–157, 162, 163, 165, 166,
193, 194, 203, 205, 207, 213, 228, 243,
250, 259, 260, 262, 266, 276, 289, 290,
306, 318, 319, 354, 355, 357, 359, 361
genes and proteins........................................207, 276,
278, 280–278, 281
AgrA...............................................................90, 91
AgrB..................................................................... 90
AgrC..............................................................90, 94
AgrD..............................................................90, 95
BviI..................................................................... 193
BviR................................................................... 193
CciI.................................................................... 193
CciR................................................................... 193
CepI..........................................................193, 194
CepR.................................................................. 193
CprB...........................................................131–142
cviI..................................................................... 6, 7
cviR..............................................................6, 9, 21
inhibitor (QSI).......................5, 8,207, 275–284,
353–361, 363–370
QSI monitor screen................................207, 276,
278, 280–278, 281
lasI..............................................25,203, 289, 290
lasR.............................................. 25, 26,156, 203,
207, 213, 228, 276, 289, 290
lux box .....................................146, 151, 259, 260
luxI...................................................4, 6, 7, 17, 18,
36, 38, 41, 145, 146, 149, 150, 203, 207, 260,
262, 266374
QUORUMSENSING
Index