RNA Detection

6. Remove slides from Coplin jars and gently tap on a stack of
   paper towels to displace excess liquid.


7. Place slides in your humidified chamber and add sufficient
   AMP 1 solution to cover all sections. Incubate at 40C for
   30 min.


8. Wash slides as described instep 5. Remove excess water by
   tapping slides on a stack of paper towels.


9. Place slides into the humidified chamber and add AMP 2 solu-
   tion to cover all sections. Incubate at 40C for 15 min.


10. Wash slides in Coplin jars using the same procedure as
    described instep 5.


11. Place washed slides in humidified chamber and add sufficient
    AMP 3 solution to cover all sections. Cover the chamber with
    the Tupperware lid and Incubate at 40C for 30 min.


12. Wash slides with 1PBT buffer in Coplin jars at room temper-
    ature. Agitate by moving slides up and down during the 2 min
    wash. Repeat this procedure for a total of three washes.


13. Remove excess liquid by tapping slides on a stack of paper
    towels.


14. Return washed slides to humidified chamber and add enough
    AMP 4 solution to cover all sections. Close the chamber and
    incubate for 15 min at 40C.


15. Wash slides with 1PBT buffer in Coplin jars at room temper-
    ature. Agitate by moving slides up and down during the 2 min
    wash. Repeat this wash for a total of three times.


16. Remove excess liquid by tapping slides on a stack of paper
    towels.


17. Return washed slides to humidified chamber and add enough
    AMP 5 solution to cover all sections. Close chamber and
    incubate for 30 min at room temperature.


18. Wash slides with 1PBT buffer in Coplin jars at room temper-
    ature. Agitate by moving slides up and down during the 2 min
    wash. Repeat this wash for a total of three times.


19. Remove excess liquid by tapping slides on a stack of paper
    towels.


20. Place washed slides into humidified chamber and add enough
    AMP 6 solution to cover all sections. Cover the chamber and
    incubate for 15 min at room temperature.


21. Wash slides with 1PBT buffer in Coplin jars at room temper-
    ature. Agitate by moving slides up and down during the 2 min
    wash. Repeat this wash for a total of three times.


22. Remove excess liquid by tapping slides on a stack of paper
    towels.

204 Viravuth P. Yin