374 17 Enzymatic Synthesis and Modification of Glycolipids
Table 5.
Protease-catalyzed glycolipid syntheses.
Protease
Solvent
Log
P
Lipid reactand
Sugar
Reference
Protease N
Dimethylformamide
–1.00
Trifluoroethylcaprylate
Sucrose
Carrea et al., 1989
Alcalase
Pyridine
+ 0.71
Vinyl laurate
Sucrose
Soedjak and Spradlin, 1994
Subtilisin Carlsberg
1
Dimethylformamide
–1.00
Trichloroethyl caprylate
Sucrose
Plou et al., 1995
SubtilisinCLEC
Pyridine
+ 0.71
Vinyl laurate
Sucrose
Polat et al., 1997
Trypsin (Porcinepancreas; modified)
Dimethylformamide
–1.00
C
18 : 1
Glucose
Ampon et al., 1991
Streptomyces
sp.
Dimethylformamide
–1.00
2,2,2-Trichloroethylbutyrate,Divinyl adipate
Glucose
Shibatani et al., 1997
Streptomyces
sp.
Dimethylsulfoxideor dimethylformamide
Divinyl adipate
Glucose, Mannose,Galactose,
a
-Methyl
D-galactose
Kitagawa et al., 1999
1
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