- DAPI is a cell permeable fluorescent probe for DNA. It allows
nuclear labeling. - Once adenoviral construct is used, several biosafety precautions
should be taken. All work with adenovirus minimally requires
BSL-2 containment. People must wear protective equipment
to reduce mucosal or skin exposure. Working surfaces must be
disinfected after use. Wastes must be disinfected by bleaching
and/or autoclave. - The optimal temperature for luciferase activity measurement is
room temperature. - ERK1/2 phosphorylation is acutely and only temporarily sti-
mulated by pro-hypertrophic stimulation. This stimulation
tempts to disappear after longer incubation time such as 24 h.
Acknowledgments
Authors are supported by grants from Fonds National de la
Recherche Scientifique et Me ́dicale (FNRS), Belgium, and Action
de Recherche Concerte ́e de la Communaute ́Wallonie-Bruxelles,
Belgium, and by unrestricted grants from AstraZeneca. F.M. is
supported by the Fund for Scientific Research in Industry and
Agriculture, Belgium. S.H. is Research Associate and L.B. is Senior
Research Associate of FNRS, Belgium. C.B. is Postdoctorate Clini-
cal Master Specialist of the FNRS, Belgium.References
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