factor 2 kinase and to its phosphorylation at a
novel site, serine 398. J Biol Chem 279
(13):12220–12231. https://doi.org/10.
1074/jbc.M309773200- Johanns M, Pyr Dit Ruys S, Houddane A,
Vertommen D, Herinckx G, Hue L, Proud
CG, Rider MH (2017) Direct and indirect
activation of eukaryotic elongation factor
2 kinase by AMP-activated protein kinase.
Cell Signal 36:212–221.https://doi.org/10.
1016/j.cellsig.2017.05.010 - Inoki K, Zhu T, Guan KL (2003) TSC2 med-
iates cellular energy response to control cell
growth and survival. Cell 115(5):577–590 - Gwinn DM, Shackelford DB, Egan DF, Mihay-
lova MM, Mery A, Vasquez DS, Turk BE, Shaw
RJ (2008) AMPK phosphorylation of raptor
mediates a metabolic checkpoint. Mol Cell 30
(2):214–226. https://doi.org/10.1016/j.
molcel.2008.03.003 - Horman S, Beauloye C, Vertommen D, Vano-
verschelde JL, Hue L, Rider MH (2003) Myo-
cardial ischemia and increased heart work
modulate the phosphorylation state of eukary-
otic elongation factor-2. J Biol Chem 278
(43):41970–41976. https://doi.org/10.
1074/jbc.M302403200 - Demeulder B, Zarrinpashneh E, Ginion A,
Viollet B, Hue L, Rider MH, Vanoverschelde
JL, Beauloye C, Horman S, Bertrand L (2013)
Differential regulation of eEF2 and p70S6K by
AMPKalpha2 in heart. Biochim Biophys Acta
1832(6):780–790.https://doi.org/10.1016/
j.bbadis.2013.02.015 - Chan AY, Dolinsky VW, Soltys CL, Viollet B,
Baksh S, Light PE, Dyck JR (2008) Resveratrol
inhibits cardiac hypertrophy via AMP-activated
protein kinase and Akt. J Biol Chem 283
(35):24194–24201. https://doi.org/10.
1074/jbc.M802869200 - Chan AY, Soltys CL, Young ME, Proud CG,
Dyck JR (2004) Activation of AMP-activated
protein kinase inhibits protein synthesis asso-
ciated with hypertrophy in the cardiac myocyte.
J Biol Chem 279(31):32771–32779.https://
doi.org/10.1074/jbc.M403528200- Li HL, Yin R, Chen D, Liu D, Wang D,
Yang Q, Dong YG (2007) Long-term activa-
tion of adenosine monophosphate-activated
protein kinase attenuates pressure-overload-
induced cardiac hypertrophy. J Cell Biochem
100(5):1086–1099. https://doi.org/10.
1002/jcb.21197 - Fujita K, Maeda N, Sonoda M, Ohashi K,
Hibuse T, Nishizawa H, Nishida M, Hiuge A,
Kurata A, Kihara S, Shimomura I, Funahashi T
(2008) Adiponectin protects against angioten-
sin II-induced cardiac fibrosis through activa-
tion of PPAR-alpha. Arterioscler Thromb Vasc
Biol 28(5):863–870. https://doi.org/10.
1161/ATVBAHA.107.156687 - Louch WE, Sheehan KA, Wolska BM (2011)
Methods in cardiomyocyte isolation, culture,
and gene transfer. J Mol Cell Cardiol 51
(3):288–298. https://doi.org/10.1016/j.
yjmcc.2011.06.012 - Belge C, Hammond J, Dubois-Deruy E,
Manoury B, Hamelet J, Beauloye C, Markl A,
Pouleur AC, Bertrand L, Esfahani H, Jnaoui K,
Gotz KR, Nikolaev VO, Vanderper A,
Herijgers P, Lobysheva I, Iaccarino G,
Hilfiker-Kleiner D, Tavernier G, Langin D,
Dessy C, Balligand JL (2014) Enhanced
expression of beta3-adrenoceptors in cardiac
myocytes attenuates neurohormone-induced
hypertrophic remodeling through nitric oxide
synthase. Circulation 129(4):451–462.
https://doi.org/10.1161/
CIRCULATIONAHA.113.004940 - Wang C, Li L, Zhang ZG, Fan D, Zhu Y, Wu
LL (2010) Globular adiponectin inhibits
angiotensin II-induced nuclear factor kappaB
activation through AMP-activated protein
kinase in cardiac hypertrophy. J Cell Physiol
222(1):149–155. https://doi.org/10.1002/
jcp.21931
342 Florence Mailleux et al.