- Be careful while adding the hydrolysis enzyme mix. Make sure
to add the 2μl inside the solution and not on the side and mix
well. Quick plate centrifugation is recommended to bring
down the solution inside the wells. Proceed quickly at this
step to avoid long exposure to light. - Prepare the reading parameters during the 30 min develop-
ment. Be careful with the choice of the plate on the ELISA
reader machine. It is recommended to take two consecutive
readings and average both results. - Normalizing to accurate protein measurements is very impor-
tant. Error in the levels of protein content highly influences the
results.
Acknowledgments
We acknowledge salary support to E.P. from the Rolande and
Marcel Gosselin Graduate Studentship and the CIHR/FRSQ
training grant in cancer research of the McGill Integrated Cancer
Research Training Program (MICRTP). The work was supported
by grants to A.P. from the Myrovlytis Trust, the Terry Fox Research
Foundation, and the Kidney Foundation of Canada.References
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