Cannabinoids

352 B. Szabo and E. Schlicker

Table 5.

(continued

)

Neurotransmitter Species

Tissue

Method

Identification of endogenous tone

1

Reference(s)

Acetylcholine

Mouse

Colon

Electrophysiology (intracellularrecording)

Inverse agonist (SR 141716), knockoutmouse generated by Marsicano et al. 2002

Storr et al. 2004

Acetylcholine,substance P

Guinea-pig Small intestine

Contraction

Inverse agonist (SR 141716)

Pertwee et al. 1996b;Coutts and Pertwee 1997;Izzo et al. 1998

Acetylcholine

Guinea-pig Ileum

(^3) [
H]Acetylcholine release
Inverse agonist (SR 141716)
Mang et al. 2001
Substance P
Mouse
Spinal cord
Substance P release
Inverse agonist (SR 141716)
Lever and Malcangio 2002
1 Inverse and partial agonists and CB
receptor disruption increase, whereas inhibition of endocannabinoid reuptake 1
or degradation decreases, transmitter release.