Science - USA (2019-01-04)
to promote these responses (fig. S6C). Thus, the poised type 2 immune potential of commensal- specific TC17 cells allows for loc ...
Biosciences.Gata3fl/fl( 37 ),Foxp3YFP-Cre( 38 )and Il17aCre( 26 ) have been previously described and were generously provided by ...
6 and 35 weeks of age were used for each experiment. Commensal culture and colonization Staphylococcus epidermidisNIHLM087 ( 44 ...
andenrichedforbead-boundcellsonmagnetized columns. RNA-sequencing and transcriptome analysis T cells were isolated by flow cytom ...
V. K. Ridauraet al., Contextual control of skin immunity and inflammation byCorynebacterium.J. Exp. Med. 215 , 785– 799 (2018). ...
RESEARCH ARTICLE SUMMARY ◥ NEUROSCIENCE Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and fo ...
RESEARCH ARTICLE ◥ NEUROSCIENCE Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting ...
in response to AMPA and NMDA stimulation ( 27 ), we performed an additional assay to test internalization of Syt3. We expressed ...
Awasthiet al.,Science 363 , eaav1483 (2019) 4 January 2019 3of14 intensity (%) time (s) NH 4 Cl NMDA intensity (%) AMPA time (s) ...
receptors [Fig. 3, A to C, and fig. S4, A to D; Syt3 short hairpin RNA (shRNA) knockdown valida- tion is provided in fig. S4, E ...
Basal synaptic transmission was also unchanged in Syt3 knockouts. Recordings of synaptic re- sponses from CA1 neurons, elicited ...
in subsequent probe tests gives a readout of spatial memory. We tested four groups simulta- neously: wild-type mice and Syt3 kno ...
Awasthiet al.,Science 363 , eaav1483 (2019) 4 January 2019 7of14 A (^1234567891011121314) 25 30 35 40 45 50 proximity to platfor ...
well (Fig. 5J) and spent the majority of their time exploring the target hole region in probe tests (Fig. 5K, top). Beginning on ...
ofwild-typemice(fig.S8B),wequantifiedprox- imity savings in hidden platform training (Fig. 6A). In the first 4 days of training, ...
then incubating for an additional 20 min, before adding the mixture to wells of a 24-well plate. Immunohistochemistry Acute hipp ...
4°C. The pellet was resuspended in 320 mM sucrose, 5 mM HEPES, pH 8. For trypsin cleavage, a 0.1 mg/ml trypsin stock solution wa ...
EPSC is considered to be purely NMDA receptor- mediated, since the measured AMPA receptor EPSC decay time constantt≈20 ms. The i ...
from the pool center (11 in an outer ring and 5 in an inner ring) over 16 days. Mice underwent 4 trials of 2 min every day. The ...
P. V. Migueset al., PKMzeta maintains memories by regulating GluR2-dependent AMPA receptor trafficking.Nat. Neurosci. 13 , 630 ...
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