Science - USA (2019-01-18)
Gaoet al.,Science 363 , eaau8302 (2019) 18 January 2019 9of16 Fig. 6. Long-range tracing and stereotypy of neuron bundles in Dro ...
four layer V neurons that could be traced to the white matter increased with increasing distance from the soma (Fig. 5C and fig. ...
Gaoet al.,Science 363 , eaau8302 (2019) 18 January 2019 11 of 16 Fig. 7. Whole-brain analysis of presynaptic sites and DANs in D ...
individual cells within the cluster (Fig. 6F, figs. S20 and S21, Movie 8, table S5, and movie S4). Although tracing of fine proc ...
( 8 , 9 )andtheultrahighresolution of correspond- ing EM pipelines ( 5 , 70 , 83 ). With genetically targeted cell type–specific ...
into a custom file format (N5) ( 21 )thatenabled parallel blockwise export and compression on a HPC cluster. Bindings for N5 for ...
U. Thomas, Modulation of synaptic signalling complexes by Homer proteins.J. Neurochem. 81 , 407–413 (2002). doi:10.1046/j.1471- ...
movies.Competing interests:Portions of the technology described here are covered by U.S. patent 7,894,136 issued to E.B., assign ...
RESEARCH ARTICLE SUMMARY ◥ GENE THERAPY CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsu ...
RESEARCH ARTICLE ◥ GENE THERAPY CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficienc ...
Sim1has a well-characterized promoter ( 30 ) and distant and robust hypothalamic enhancer (~270 kb from the transcription start ...
Matharuet al.,Science 363 , eaau0629 (2019) 18 January 2019 3of11 Fig. 1. CRISPRaSim1up-regulation in vitro and obesity rescue i ...
Matharuet al.,Science 363 , eaau0629 (2019) 18 January 2019 4of11 Fig. 2.dCas9andSim1mRNA expression levels in CRISPRa trans- ge ...
only in theSim1promoter for Prm-CRISPRa and SCE2 in Enh-CRISPRa mice (fig. S7, C and D), similar to what we observed in the ChIP ...
dCas9–fused VP64 vector, which has an AAV packaging load of 4.3 kb (Fig. 3A;pCMV-sadCas9- VP64). AsS. aureususes a different pro ...
Using CRISPRa targeting for either the pro- moterorenhancerofSim1,wecouldrescuethe obesity phenotype in a tissue-specific manner ...
size during development that is observed in Sim1+/−mice ( 22 ) or by disturbed energy ho- meostasis during adulthood was largely ...
harvested 48 hours after transfection and 5 days after infection to isolate RNA for qRT-PCR analysis. RNA isolation, RNA-seq, an ...
injections carried out in a previous study ( 56 ). A 0.5-mm hole was created in the cranium with a high-speed model 1911 Stereot ...
R. C. Hirst, K. J. McCullagh, K. E. Davies, Utrophin upregulation in Duchenne muscular dystrophy.Acta Myol. 24 , 209– 216 (2005 ...
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