Science - USA (2019-02-15)

Behavioral tests Three-month-old mice expressing GFP (n=12 mice) or GFP-(PR) 50 (n= 11 mice) were subjected to behavioral analys ...

GE Healthcare), and the recombinant proteins were eluted, desalted, and concentrated. In vitro DPR protein and HP1aassays HP1ali ...

A. R. Stromet al., Phase separation drives heterochromatin domain formation.Nature 547 , 241–245 (2017). doi:10.1038/ nature229 ...

RESEARCH ARTICLE SUMMARY ◥ STRUCTURAL BIOLOGY Recognition of the amyloid precursor protein by humang-secretase Rui Zhou, Guanghu ...

RESEARCH ARTICLE ◥ STRUCTURAL BIOLOGY Recognition of the amyloid precursor protein by humang-secretase Rui Zhou^1 , Guanghui Yan ...

( 30 ), is dynamic and disordered in substrate-free g-secretase ( 31 ) but becomes ordered upon bind- ingtothesubstrateandcontri ...

mutations, which result in abrogation of APP- C99 cleavage in vitro by the corresponding g- secretase variants ( 20 ). APP recog ...

PS1 are employed to interact with the TM helix from APP and Notch. Leu^85 , Thr^147 , and Ile^287 contribute to APP but not Notc ...

the active site (Fig. 5D). The second group of 19 residues has side chains pointing into the substrate-binding pore (Fig. 5D). T ...

through a pair of conserved H-bonds, and sub- strate cleavage is oriented through formation of a hybridbsheet between the substr ...

extracellular domain, a TM region, and a short intracellular domain known as AICD (fig. S1A). On the basis of sequence alignment ...

J. A. Hardy, G. A. Higgins, Alzheimer’s disease: The amyloid cascade hypothesis.Science 256 , 184–185 (1992). doi:10.1126/scien ...

RESEARCH ARTICLE SUMMARY ◥ TOXINS The human gut bacterial genotoxin colibactin alkylates DNA Matthew R. Wilson, Yindi Jiang, Pet ...

RESEARCH ARTICLE ◥ TOXINS The human gut bacterial genotoxin colibactin alkylates DNA Matthew R. Wilson^1 †, Yindi Jiang^1 , Pete ...

an observed DNA adduct can allow for the de- termination of its elemental composition, and the triggered MS^2 and MS^3 fragmenta ...

m/z552.2125 [M+H]+( 5 )andm/z568.2076 [M+H]+( 6 and 7 ) (figs. S30 and S39). MS^2 frag- mentation confirmed that these compounds ...

leads to DNA damage. The precolibactins, iso- lated to date, possess a variety of cyclopropane- containing scaffolds, including ...

reported the accumulation of a putative inter- strand cross-link in DNA incubated withpks+ E. colias assessed by gel electrophor ...

N. Bossuet-Greifet al.,Escherichia coliClbS is a colibactin resistance protein.Mol. Microbiol. 99 , 897–908 (2016). doi:10.1111 ...

RESEARCH ARTICLE ◥ STRUCTURAL BIOLOGY A human postcatalytic spliceosome structure reveals essential roles of metazoan factors fo ...