Nature - USA (2020-08-20)

5 mg ml−1 yeast in 5% sucrose solution that was deposited on filter-paper discs (Whatmann) and exchanged every 24–30 h. For P. e ...

Article phalloidine633 1:10,000. After two washes for 10 min in 0.15% PBST, ovaries were mounted on slides with Vectashield. Ima ...

Reporting summary Further information on research design is available in the Nature Research Reporting Summary linked to this pa ...

Article Extended Data Fig. 1 | See next page for caption. ...

Extended Data Fig. 1 | 20HE feeding promotes sexually dimorphic ISC mitotic activity. a, Male ISCs do not divide strongly in res ...

Article Extended Data Fig. 2 | See next page for caption. ...

Extended Data Fig. 2 | The second mitotic wave of 20HE requires EcR–Usp in progenitors whereas EcR is dispensable to ISCs in the ...

Article Extended Data Fig. 3 | See next page for caption. ...

Extended Data Fig. 3 | The second mitotic wave of 20HE regulates Jak–Stat signalling and requires Egfr signalling in the midgut ...

Article Extended Data Fig. 4 | Long-term 20HE feeding promotes sexually dimorphic ISC division and gut growth. a, 1 mM 20HE feed ...

Extended Data Fig. 5 | See next page for caption. ...

Article Extended Data Fig. 5 | Mating requires ecdysteroidogenic enzymes from the early ovarian follicles and escort cells to in ...

Extended Data Fig. 6 | See next page for caption. ...

Article Extended Data Fig. 6 | Ovaries of the esg-Gal4ts, esgts Su(H)-Gal80 midgut drivers have GFP expression in their germaria ...

Extended Data Fig. 7 | JH receptors are required for ISC divisions, and exogenously fed JH inhibits ISC mitoses in response to o ...

Article Extended Data Fig. 8 | See next page for caption. ...

Extended Data Fig. 8 | Eip75B is a downstream ecdysone-inducible effector required to stimulate ISC proliferation, through Hr3 r ...

Article Extended Data Fig. 9 | Nitric oxide modulates the interaction of Eip75B and Hr3 to regulate ISC division. a–d, Eip75B is ...

Extended Data Fig. 10 | See next page for caption. ...

Article Extended Data Fig. 10 | Ovary-derived 20HE promotes intestinal dysplasia through EcR, Usp and Eip75B, which may affect D ...