Plant Biotechnology and Genetics: Principles, Techniques and Applications

7.3.1.2. CreatorTMCloning.The Creator cloning system is an alternative approach that allows the efficient transfer of DNA fragme ...

system makes use of the Cre-loxPsite-specific recombination system to transfer DNA frag- ments from a reference plasmid, known a ...

7.4 Vector Design Recombinant DNA technology has made an enormous impact on plant biotechnology, both in the development of nove ...

new challenges in the production and analysis of recombinant DNA. Large numbers of promoters and genes encoded by these genomes ...

no longer a practical approach to facilitate the analysis of all the genes and promoters from these model organisms. Plant vecto ...

revolutionized gene analysis by allowing genes to be expressed from the same recombina- tion cassette inE. coli, Saccharomyces c ...

7.4.5 Vectors Derived from Plant Sequences The revolutionary advances in recombinant DNA technology provide great opportunities ...

non-plant-derived sequences are kept to a minimum. The T-DNA ofAgrobacteriumis one source of foreign genetic material that could ...

crop improvement. Frequently, viral promoters, such as the CaMV 35Spromoter, are used to express genes constitutively and near-u ...

Figure 7.22.A schematic representation of six expression cassettes inserted within each of the aux- illary vectors described in ...

heavy rain and windy conditions. The dwarf plants of the so-called Green Revolution are short because they respond abnormally to ...

7.5. TARGETED TRANSGENE INSERTIONS Once a recombinant T-DNA vector has been generated, with features designed to provide stable ...

nuclear genome integration with elements for eukaryotic gene expression; and a plastid vector, with elements that allow prokaryo ...

constructed so that they contain left and right plastid-targeting regions (LTR and RTR), which are 1–2 kb in size and homologous ...

LIFE BOX 7.1. WAYNE PARROTT Wayne Parrott, Professor, Department of Crop and Soil Sciences, University of Georgia Wayne Parrott ...

But, research is also about people. The value of those who have served as my mentors along the way cannot be under- stated. I mu ...

Karimi M, Inze D, Depicker A (2002): GATEWAY vectors for Agrobacterium-mediated plant trans- formation.Trends Plant Sci 7 :193–1 ...

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&CHAPTER 8 Genes and Traits of Interest for Transgenic Plants KENNETH L. KORTH Department of Plant Pathology, University of ...

is supported by many people and organizations, there are also some who oppose the technology. Considering their possible motivat ...