Plant Biotechnology and Genetics: Principles, Techniques and Applications

&CHAPTER 11 Transgenic Plant Analysis JANICE ZALE Department of Plant Sciences, University of Tennessee, Knoxville, Tennesse ...

11.1 Introduction After a particle bombardment orAgrobacterium-mediated transformation experiment to generate transgenic plants ...

actually occurred. Gene expression may be due not to stable integration of a transgene, but due to transient expression (Bellucc ...

events. Therefore, if 100 T 1 millet seeds segregating for a single insertion are plated onto tissue culture media containing a ...

Extra precautions should be taken when assaying putative transformants produced using Agrobacterium-mediated gene transfer as th ...

hydrogen bonds (Memelink et al. 1994; Sambrook and Russell 2001). The wells are washed and incubated with an enzyme-linked conju ...

placed on the gel and the DNA molecules migrate upward into the membrane via capillary action. However, a more recent protocol d ...

are desirable because of simple segregation patterns, among other reasons. As an example, Figure 11.6 shows a plasmid used inAgr ...

marker (SM) probe (Fig. 11.7). Copy number can be determined relative to either T-DNA border, but right border analysis is the b ...

11.4.2 Determining the Presence of Intact Transgenes or Constructs DNA gel blots are also used to determinewhethera particular t ...

Nonspecifically bound probe is washed from the membrane (Memelink et al. 1994; Sambrook and Russell 2001), and the membrane is e ...

One of the most important aspects of specific transcript quantitation is use of the appropriate statistical analyses (Yuan et al ...

dodecyl sulfate polyacrylamides (SDS-PAGE) gels are most commonly used for separating proteins on the basis of size (Laemmli 197 ...

will demonstrate transgene expression. Phenotypic traits other than the GOI may be altered in a transformed plant. References Be ...

Memelink J, Swords KMM, Staehelin LA, Hoge JHC (eds) (1994):Southern, Northern and Western Blot Analysis. Kluwer Academic Publis ...

...

&CHAPTER 12 Regulations and Biosafety ALAN McHUGHEN University of California, Riverside, California 12.0. CHAPTER SUMMARY AN ...

getting enough to eat, but also a range of other concerns, such as food safety and nutrition. In addition, other economic and po ...

(GEOs or GMOs). In response to this, governments around the world rushed to assure the public that “something was being done to ...

(environmental risks) by USDA. This coordinated effort and allocation of responsibility to different agencies continues today in ...