Science - USA (2021-07-16)

serum of old mice (Fig. 1C). To confirm the effect of LPS on aged mice with an increased SnC burden, we also treated progeroidEr ...

Camellet al.,Science 373 , eabe4832 (2021) 16 July 2021 3 of 12 Fig. 2. The SARS-CoV2 spike protein-1 (S1) exacer- bates the sec ...

To address specifically how the SARS-CoV-2 PAMPs affect SnCs, senescent human kidney endothelial cells (fig. S4) were treated wi ...

these data suggest that S1 is a PAMP that can trigger a hyperinflammatory state in SnCs, possibly through stimulation of a Toll- ...

Camellet al.,Science 373 , eabe4832 (2021) 16 July 2021 6 of 12 A B C D Young + Vehicle Young + Fisetin Old + Vehicle Old + Fise ...

in the old than young mice (Fig. 4C), which is consistent with the premature death of the old mice. However, in old mice treated ...

ofp16Ink4a-expressing SnCs ( 42 ).INK-ATTAC mice express a caspase 8-FKBP fusion protein, ATTAC( 43 ), from thep16Ink4apromoter. ...

similar to that in COVID-19 patients. MHV was a primary viral pathogen transferred by the NME as evidenced with serology, quan- ...

andClostridium piliforme. Relative serology scores for MHV antigens (recombinant A59- strain nucleocapsid protein, purified A-59 ...

RNA extraction Tissues were snap-frozen after harvest. RNA was extracted using Trizol after homogeniza- tion in a bead beater. A ...

C. Lucaset al., Longitudinal analyses reveal immunological misfiring in severe COVID-19.Nature 584 , 463–469 (2020). doi:10.103 ...

RESEARCH ARTICLE SUMMARY ◥ MICROBIOLOGY A human apolipoprotein L with detergent-like activity kills intracellular pathogens Ryan ...

RESEARCH ARTICLE ◥ MICROBIOLOGY A human apolipoprotein L with detergent-like activity kills intracellular pathogens Ryan G. Gaud ...

coating occurred in unprimed cells, indicating that cytokine activation was needed for APOL3 expression but not its relocation. ...

Stmand reversed inDAPOL3cells treated with IFN-g(Fig. 2D and movies S3 and S4). Stmlacking the Cpx-driven IM repair path- way (S ...

were accompanied by a marked transformation of the APOL3 bacterial coat: Superresolution structured illumination microscopy (SIM ...

rAPOL3-induced killing in each case (Fig. 3C). S. flexneri,B. thailandensis,Escherichia coli, andL. monocytogeneswere likewise s ...

sufficiently to capture lipid extraction and blebbing by live confocal imaging of giant unilamellar vesicles (GUVs) and negative ...

rAPOL3) upon killing bacteria and were only evident at a sufficiently high mass spectral col- lisional activation (HCD) energy t ...

cholesterol-poor anionic membranes ( 36 – 38 ). Notably, organelles such as mitochondria also contain some of the same anionic l ...