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Table 11.4Stationary phases commonly used for exclusion chromatography Polymer Trade name Fractionation rangea (Mr 10 –3) Low-p ...

Viruses, enzymes, hormones, antibodies, nucleic acids and polysaccharides have all been separated and purified by use of appropr ...

centrifugation in that it does not rely on differences in the physical properties of the analytes. Instead, it exploits the uniq ...

enzyme is added progressively to the insolubilised ligand in a column, the enzyme molecules will be stimulated to bind and a dyn ...

for the isolation any GST-tagged cloned protein. It is possible to remove the GST tag in a one-step process by adding PreScissio ...

Applications Many enzymes and other proteins, including receptor proteins and immunoglobulins, have been purified by affinity ch ...

(Section 6.7.1). The cobalt commercial product called Dynabeads Myone TALON™has the practical advantage that it only binds adjac ...

between a stationary liquid phase and a mobile gas phase of the volatilised analytes as they are carried through the column by t ...

volatilisation. The stationary phase consists of a high-boiling-point liquid material such as silicone grease or wax that is eit ...

Apiezon L (non-polar) and esters of adipic, succinic and phthalic acids.b-Cyclodextrin- based phases are available for chiral se ...

retained on the column for excessive periods of time if they are applied directly. Poor resolution and peak tailing usually acco ...

analyteþH 2 þO 2 !combustion productsþH 2 Oþionsþradicalsþelectrons X ðionsÞþ X ðelectronsÞ!current Nitrogen–phosphorus detec ...

Mass spectrometer detector: This is a universal detector that gives a mass spectrum of the analyte and therefore gives both str ...

12 Spectroscopic techniques: I Spectrophotometric techniques A. HOFMANN 12.1 Introduction 12.2 Ultraviolet and visible light spe ...

12.1.1 Properties of electromagnetic radiation The interaction of electromagnetic radiation with matter is aquantum phenomenon a ...

a particle, albeit having a mass of zero. As a particle, it interacts with matter by transferring its energyE: E¼ hc l ¼h ð 12 ...

Electronic ground state (S 0 ) Electronic excited state (S 1 ) Nuclear displacement m m v=0 hn DH Re(ground) v=1 v=2 v=3 v=4 v=5 ...

The plot of absorption probability against wavelength is calledabsorption spectrum. In the simpler case of single atoms (as oppo ...

orbitals. Otherporbitals combine to form non-bindingnorbitals. The population of binding orbitals strengthens a chemical bond, a ...

Table 12.1Common colorimetric and UV absorption assays Substance Reagent Wavelength (nm) Amino acids (a) Ninhydrin 570 (proline ...