492 INDEX
Magnetic circular dichroism (MCD)
spectroscopy, 40, 77, 130, 374, 452
Magnetic measurements, see also
electron paramagnetic resonance
(EPR), 17
Magnetic receptivity, 99
Manganese (Mn), 2, 2t, 4t, 7t, 11t, 18,
361
electron transfer and, 23
group I introns and, 243, 250, 261, 272
hammerhead ribozyme and, 267t, 269,
270–271t, 280–290
structure and triggers, 4t
Marcus theory, 25–26, 415
Mass spectrometry (MS), 76, 142,
14 8 –153
cytochrome c oxidase models and,
440
electrospray ionization-MS (ES-MS),
382
instrumentation, 149
MALDI-TOF MS, 43, 149–150, 152,
441
peptide and protein sequencing and,
150–153
protein folding and, 149
proteomics and, 40, 42–43, 150–153
Matrix metalloproteinase, 119–120,
121f
Maxam-Gilbert sequencing method, 59,
62
M center (nitrogenase), 137–138, 137f
Mechanisms, see outer sphere, inner
sphere, interchange, associative,
dissociative, and enzyme of
interest
Medicinal chemistry, 14, 73, 190t
polymerase chain reaction (PCR)
and, 61
MEDLINE, 184 –185
Messenger RNA (mRNA), 54–56, 58–
59, 239, 458
Metal chelators, 8, 32, 47, 81f
Metal ion complexation:
kinetics, 9–13
thermodynamics, 8–9
Metal-ligand interactions, 6, 25
Metal-metal bonds, 21–22
Metallocenes, 19–20
Metalloenzyme systems, see also
individual proteins, 22, 27, 30, 44,
73
Metalloproteins: see also individual
proteins
characteristics of, 1, 14, 27, 30, 60, 73
electron exchange, 24
isolation, 353
model compounds and, 352–353
modeling and, 166
purifi cation, 84, 353
Metallothionein, 174
Metals, in biological systems, 3–6
Metal toxicity, 2
Methane monooxygenase, 22, 459– 463
electron transfer and, 461–463
EPR studies, 460 –464
EXAFS studies, 461–464
Particulate methane monooxygenase
(pMMO), 460–465
soluble methane monooxygenase
(sMMO), 460–463
X-ray crystallography, 461–465, 463f
Methionine (Met, M), 22, 31f, 33f, 40,
56
calmodulin and, 304, 317, 322, 324
cytochrome c iron ligand and, 117,
408, 410 – 411
cytochrome c oxidase copper ligand
and, 131, 429, 430, 463
Michaelis constant, 45
Michaelis- Menten enzyme kinetics, 13,
45–47, 326
competitive inhibition, 46–47, 46f
noncompetitive inhibition, 46–47, 46f
rate equation, 45
stopped-fl ow kinetics, 143 –14 4, 354,
419, 421, 437
MM2/MM3 force fi eld, 161, 164, 166,
178
M N DO, 161, 174 –176, 179
M N DO /d, 174, 179
Model compounds, see individual
proteins
Molecular modeling (MM), 158–159,
16 0 –170, 178 –18 0, 182
biomolecule modeling, 166 –167
characteristics of, 160 –164
cytochrome bc 1 and, 167–170