Science - USA (2021-12-10)
repair.Nat. Commun. 12 , 3338 (2021). doi:10.1038/ s41467-021-23684-x; pmid: 34099686 T. Riedl, F. Hanaoka, J.-M. Egly, The com ...
M. J. Dunham, S. D. Emr, F. Hughson, J. Hurley, K. Murakami, N. Nakamura, E. Nogales, R. Schekman, S. Shan, S. Showman, K. Sugas ...
RESEARCH ARTICLE SUMMARY ◥ CELL BIOLOGY Nuclear pores dilate and constrict in cellulo Christian E. Zimmerli†, Matteo Allegretti† ...
RESEARCH ARTICLE ◥ CELL BIOLOGY Nuclear pores dilate and constrict in cellulo Christian E. Zimmerli1,2,3†, Matteo Allegretti1,3† ...
fig. S4B, and movie S1) as in vertebrates and algae but as opposed to the single ring ob- served inS. cerevisiae(fig. S3A) ( 4 , ...
in preparations of isolated NEs or NPCs but may also be reduced within intact cells—e.g., during stress conditions. We first set ...
spokes. By contrast, in the constricted state, the spokes form extensive contacts (Fig. 4, A and B) equivalent to those in the p ...
Thus, it appears more likely that NPC diam- eter changes result from mechanical forces that are applied externally. Membrane ten ...
of individual tomograms, the average NPC diameter correlated strongly with the average INM-ONM distance (Spearman correlation co ...
such as the energy status of the cell or ex- posure to OS. Notably, ED resulted in a large heterogeneity of NPC constriction lev ...
Zimmerliet al.,Science 374 , eabd9776 (2021) 10 December 2021 8 of 15 3456 40 50 60 70 80 Nuclear volume (m^3 ) N PC di amete r( ...
(YES-broth from Formedium +20 g agarose/L) at 30°C for maximum 3 days and re-streaked on fresh YES-plates before liquid culture ...
40 min before cryo-EM grid preparation, as described above. Live-cell fluorescence microscopy and FRAP imaging Ibidim-Slide eigh ...
data acquisition on energy-depleted cells i.e., 210 min after sample preparation. To measure cytoplasmic diffusion, FRAP data- ...
align program dropped below a threshold of The final aligned TS were reconstructed using the SIRT-like filtering option in IMOD ...
NPC diameter and volume measurements NPC diameters were measured with an in- house MATLAB script based on the final co- ordinate ...
Step 2 The above steps led to ensembles of separate models for the cytoplasmic side, NR, and IR. To build the model of the full ...
S. Abuhattumet al., Intracellular Mass Density Increase Is Accompanying but Not Sufficient for Stiffening and Growth Arrest of ...
Yes, I would like more informationaboutjoiningthe AAAS 1848 Society. PLEASECONTACT MEAT: Name: Address: City: State: Zip code: C ...
RESEARCH ARTICLE SUMMARY ◥ NEURODEVELOPMENT Mouse and human share conserved transcriptional programs for interneuron development ...
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