Science - USA (2021-12-10)
PI(3,4)P 2 levels at the midbody as well as elon- gated intercellular bridges (Fig. 4, F and G, and fig. S14A). Removal of TSG10 ...
(Fig. 4H), but overexpression of VPS36-mCherry increased CHMP4B-GFP localization (fig. S15A). Thus, VPS36/ESCRT-II participates ...
relatively promiscuously to phosphoinositides, including PI(3,4)P 2 ( 13 , 14 ). The molecular ex- planation for this evolutiona ...
at the intercellular bridge of other secondary ingression site effectors, such as FIP3 and F-actin, were observed (fig. S17, G a ...
recruitment of ESCRT-III at the midbody de- pends on ALIX ( 4 ), but the presence of other mechanisms that trigger ESCRT III–med ...
(gift from Emilia Turco, University of Turin, Italy), anti-Citron Kinase (Transduction Labo- ratories,BDBiosciences,FranklinLake ...
transferred to a 15 ml conical tube and centri- fugedat2800×gfor20minat4°C.Afterre- moving as much liquid as possible, the midbo ...
4_E04) were received from the International Mouse Phenotyping Consortium and used to generate chimeric mice.Pik3cafl/flmice were ...
I. Goliand, D. Nachmias, O. Gershony, N. Elia, Inhibition of ESCRT-II-CHMP6 interactions impedes cytokinetic abscission and lea ...
RESEARCH ARTICLE SUMMARY ◥ STRUCTURE PREDICTION Computed structures of core eukaryotic protein complexes Ian R. Humphreys†, Jimi ...
RESEARCH ARTICLE ◥ STRUCTURE PREDICTION Computed structures of core eukaryotic protein complexes Ian R. Humphreys1,2†, Jimin Pei ...
into multiple pieces because of errors in gene prediction, or recent gene expansions spe- cific to certain lineages. We dealt wi ...
0.219), using the highest predicted contact probability over all pairs of residues in the two proteins as a measure of the prope ...
archaeal topoisomerase VI ( 31 ), which couples adenosine 5 ́-triphosphate–dependent dimer- ization of Top6B subunits to DNA cle ...
homology models ( 27 , 31 ). Both Rec102 and Top6B have long, helical arms that feed into the Spo11 interface; our model predict ...
of Rad51 activity ( 35 ); in yeast, the Rad51 para- logs Rad55 and Rad57 form a stable homodimer that accelerates assembly of Ra ...
by a conserved complex containing Rad4 (XPC—Xeriderma pigmentosum group C pro- tein in humans), Rad23 (HR23B), and Rad33 (Centri ...
(39) suggests how the Rad14 C terminus, which fits into previously unmodeled density, interacts with TFIIH. The long central hel ...
predicted structure (Fig. 2) of their interac- tion provides clues about the mechanism of their function ( 74 ). The GARP comple ...
helices, such as single transmembrane helices or coiled coils, may be overpredicted (in ini- tial studies of human complexes, in ...
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