194 M.V. Moreno-Arribas et al.
Ta b l e 6 B. 1(continued)Sample preparationTechnique usedReferenceProcedureMaterialPeptides separationPeptides detectionDialysisSpectra POR3 membranePAGE-SDSSilver stainingMart ́ınez-Rodriguez and
Polo (2000)UltrafiltrationCut-off membrane 10,000 Da RP-HPLC Nova-Pak C18columnAbsorbance at 214 nm↓↓Low pression exclusion chromatographySephadex G-10UltrafiltrationCut-off membrane 5000 DaDos Santos et al. (2000)↓Cut-off membrane 500 DaUltrafiltrationCut-off membrane 3000 DaRP-HPLC HypercarbcolumnAbsorbance at 214 nm andfluorescence of the OPAderivativesDesportes et al. (2000)↓Cut-off membrane 200-300 Da↓CEAbsorbance at 214 nm↓↓Low pressure exclusion chromatographySephadex LH-20UltrafiltrationCut-off membrane 3000 DaRP-HPLC HypercarbcolumnAbsorbance at 214 nm andfluorescence of the OPAderivativesDesportes et al. (2001)↓Cut-off membrane 200-300 Da↓↓CEAbsorbance at 214 nm↓Low pressure exclusion chromatographySephadex LH-20UltrafiltrationCut-off membrane 5000 DaAlexandre et al. (2001)