Science - USA (2022-01-21)
connector and 7.5mM of each terminal cap. (Lower absolute concentrations with the same ratios were used for some trimers.) ABCA ...
with substrate, and immediately recorded. All kinetic measurements were fitted to a single exponential decay function: S=A*exp(− ...
665 – 680 (2020). doi:10.1038/s41592-020-0848-2; pmid: 32483333 B. Coventry, D. Baker, Protein sequence optimization with a pai ...
RESEARCH ARTICLE SUMMARY ◥ SYNTHETIC BIOLOGY Synthetic multistability in mammalian cells Ronghui Zhu, Jesus M. del Rio-Salgado, ...
RESEARCH ARTICLE ◥ SYNTHETIC BIOLOGY Synthetic multistability in mammalian cells Ronghui Zhu^1 , Jesus M. del Rio-Salgado^1 , Jo ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 2 of 11 A Ideal capabilities for a synthetic multistable circuit B Natur ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 3 of 11 mCitrine- mCitrine+ WT R39A R2AR39AR2AR39AR67A Mutations 1 10 20 ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 4 of 11 B D Time-lapse imaging reveals MultiFate-2.3 tristability High T ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 5 of 11 B External inducers switch cells between states A Modulating TF ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 6 of 11 Box 1. Design of the MultiFate circuit. Here we introduce the ma ...
Zhuet al.,Science 375 , eabg9765 (2022) 21 January 2022 7 of 11 2 3 4 5 6 7 8 9 10 11 Number of transcription factors (N) 101 10 ...
marker, into Chinese hamster ovary K1 (CHO- K1) cells and analyzed Citrine expression by flow cytometry 36 hours later (Fig. 2A ...
transferred back to the high-TMP media (Fig. 4A, bottom, and fig. S9). Thus, MultiFate’s ability to support irreversible transit ...
in a manner analogous to the stepwise expan- sion of MultiFate circuits demonstrated here ( 21 , 22 , 41 , 42 ). A remarkable fe ...
755 – 766 (2006). doi:10.1016/j.cell.2006.06.052; pmid: 16923394 T. Miyamotoet al., Myeloid or lymphoid promiscuity as a critic ...
GOLDOPENACCESS,DIGITAL,ANDFREETO ALLREADERS AsAAAS’s firstmultidisciplinary, openaccessjournal,ScienceAdvancespublishes research ...
RESEARCH ARTICLES ◥ ATTOSECOND SCIENCE Attosecond coherent electron motion in Auger-Meitner decay Siqi Li1,2†, Taran Driver1,3,4 ...
of the degenerate 2pelectrons participates in the decay, leading to excited cationic states. There is a small contribution from ...
electron emitted attiwill experience a mo- mentum shift in the direction ofA → ðÞti. Because the periodTLof the circularly polar ...
limit of this region is∼6.4 atomic units, and the upper limit extends to include all electrons at higher momenta. Equation 2 can ...
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