Science - USA (2022-01-21)
RESEARCH ARTICLE ◥ CORONAVIRUS Structural basis for continued antibody evasion by the SARS-CoV-2 receptor binding domain Katheri ...
bind human ACE2 (e.g., RaTG13 virus RBD affinity of 3.9mM) ( 30 ). The day 146 RBD, however, had a similar affinity (Kdof 46 nM) ...
showninacryo–electron microscopy (cryo-EM) structure of the SARS-CoV-2 spike protein con- taining the N501YRBDsubstitution bound ...
been noted in other reports when human cells overexpressing ACE2, as opposed to African greenmonkey(Vero)targetcells,wereusedto ...
when it comes to resistance to polyclonal antibodies. One-quarter of sampled individuals had no detectable activity against the ...
A6 activity against infectious SARS-CoV-2 in a plaque reduction neutralization assay (fig. S4B). A3 neutralized almost all SARS- ...
surface areas using molecular dynamic trajec- tories of a fully glycosylated SARS-CoV-2 spike protein with a modeled N370RBDglyc ...
virus spike protein contains a threonine at RBD position 372, which would allow for N370RBDglycosylation (Fig. 5F). Despite the ...
W. F. Garcia-Beltranet al., Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity. Cell 184 , ...
(S10 RR029205). This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science Us ...
RESEARCH ARTICLE SUMMARY ◥ PROTEIN DESIGN Reconfigurable asymmetric protein assemblies through implicit negative design Danny D. ...
RESEARCH ARTICLE ◥ PROTEIN DESIGN Reconfigurable asymmetric protein assemblies through implicit negative design Danny D. Sahtoe1 ...
enable rigid fusion at both the N and C termini (fig. S1). Heterodimers with pairedbstrands across the interface were generated ...
designed helical repeat proteins (DHRs) to ter- minal helices ( 23 , 28 ). Because these DHRs have different shapes, they also s ...
Unlike previously designed heterodimers, binding reactions equilibrated rapidly, with affinities ranging from micromolar to low ...
simultaneously and conceptually resemble Ste5 and related scaffolding proteins that organize mitogen-activated protein (MAP) kin ...
nsEM confirmed that this higher-order com- plex is similar to the designed C4-symmetric ring (Fig. 4D and fig. S21). To determin ...
disassembly of the trimer and formation of the ring (Fig. 5B and fig. S23, A to C). Because ring formation is cooperative owing ...
subunit exchange and complex reconfigura- tion in response to signal inputs for synthetic biology and other applications. Becaus ...
Plasmids encoding the individual protomers were ordered in pET29b with an N-terminal polyhistidine His6× tag followed by a TEV c ...
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