Science - USA (2022-02-04)
RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY CRISPR activation and interference screens decode stimulation responses in primary human T ...
RESEARCH ARTICLE ◥ IMMUNOLOGY CRISPR activation and interference screens decode stimulation responses in primary human T cells R ...
they could miss necessary components that wouldonlybeidentifiedthroughloss-of- function studies. We therefore performed recip- r ...
Schmidtet al.,Science 375 , eabj4008 (2022) 4 February 2022 3 of 12 TAF4TT PTPRC CD3G VVAVAV1IL2 ZAP70 CD3D CD3E LCP2 CD2CD28 CD ...
overlapped across all CRISPRa screens, likely representing core regulators of type 1 cytokine production in response to stimulat ...
Schmidtet al.,Science 375 , eabj4008 (2022) 4 February 2022 5 of 12 APOBEC3C APOBEC3D FOXQ1OO IFNG IL2 IL2RB LAT2AA MAP4K1 OTUD7 ...
Schmidtet al.,Science 375 , eabj4008 (2022) 4 February 2022 6 of 12 IFNGIL2 No Targets Control DEPDC7DEF6 TAGAPRAC2 VAV1 Signali ...
cytokine gene expression, whereas positive regulators generally promoted more diverse cytokine expression patterns than negative ...
Biolabs, catalog no. E1602L). sgRNAs have been cloned into pXPR_502 (Addgene, catalog no. 96923) for CRISPRa and into CROPseq-Gu ...
than the other screens. To address this, addi- tional fixed cells from the same experiment were stained and sorted as an additio ...
(Luminex). To remove very lowly expressed cytokines for downstream analysis, any group in which three of four donors had undetec ...
trees shown were generated using the Seurat BuildClusterTreefunction with default argu- ments. For pseudobulk differential expre ...
C. Hafemeister, R. Satija, Normalization and variance stabilization of single-cell RNA-seq data using regularized negative bino ...
RESEARCH ARTICLE SUMMARY ◥ PLANT SCIENCE Natural historyÐguided omics reveals plant defensive chemistry against leafhopper pests ...
RESEARCH ARTICLE ◥ PLANT SCIENCE Natural historyÐguided omics reveals plant defensive chemistry against leafhopper pests Yuechen ...
NaJAZi, andNaJAZjnegatively correlated with theEmpoascanumbers and damage. The expression of JAZ genes known to me- diateM. sext ...
lines ofN. attenuataplants individually RNA interference (RNAi)–silenced [inverted repeat (ir)] or overexpressed (ov) in differe ...
Baiet al.,Science 375 , eabm2948 (2022) 4 February 2022 4of9 A 0 5 10 15 20 25 30 0 0.5 1 2 3 6 9 24 0 20 40 60 80 100 120 140 1 ...
RILs (data S2) and that seemingly minor differences in leaf sampling and extraction technique—potentially the simultaneous ex- p ...
involved in the regulation and biosynthesis of CP andm/z347.19, includingNaMYC2a,NaMYB8, andNaAT1, which encodes a hydroxycinnam ...
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