BioPHYSICAL chemistry
304 PART 2 QUANTUM MECHANICS AND SPECTROSCOPY Wavelength (nm) Absorbance spectrum Absorbance (a.u.) Absorbance Fluorescence Wave ...
differences are small and not resolved, result- ing in a broad spectrum. Also, lines may be broadened due to the uncertainty pri ...
Since the rates are first order, the observed rate is given by just the sum of the rates for all possible decay pathways: (14.28 ...
then passed onto a detector where the amount of fluorescence is measured. In the case of fluorescence spectrophotometers, one ty ...
to 11 Å. Not only does each bacteriochlo- rophyll experience different interactions with the other bacteriochlorophylls but the ...
excitation, and the use of successive delays provides the time-evolution profile of the couplings. The ability of this technique ...
et al. (2005) on the FMO complex, demonstrate the feasibility of apply- ing 2D optical spectroscopy to complexbiological systems ...
In single-molecule spectroscopy, a key concept is that a single molecule can be repetitively cycled between the ground and excit ...
efficiencies, or in terms of the time dependence of the FRET efficiency, corresponding to the rate of change between the open an ...
and new DNA molecules are formed by breakage and joining of the homo- logous segments. This process is facilitated by the protei ...
is a dynamic structure that can adopt two different conformations that are in equilibrium with each other. In one conformation t ...
Problems 14.1 Write Beer’s law and define all of the terms. 14.2 What is the frequency of light with a wavelength of (a) 1 nm, ( ...
14.7 For a sample in a 1-cm cuvette, if the extinction coefficient of a molecule is 1 × 104 M−^1 cm−^1 at 500 nm, what is the ab ...
Crystallography is the most common experimental method for obtaining a detailed picture of a protein or protein complex. Such ex ...
a wave passing through a series of slits that are separated by a distance, a (Figure 15.2). Passing through a slit does not chan ...
other slits having larger path differences of 2λ, 3λ, 4λ, etc., so that all of the waves will add together constructively. Thus, ...
where nis a positive integer; this equation is known as Bragg’s law. At this angle, each of the waves from the other lattice pla ...
simplest form of these cells are the primitive cells, denoted as P, which have lattice points at each corner. The non-primitive ...
Protein crystals Proteins are only stable in the presence of the proper buffer; therefore, the crystals are always grown from so ...
slowly by vapor exchange with a large reservoir solution that is initially poised at a higher salt concentration. Since the cont ...
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