Instant Notes: Analytical Chemistry

Column reaction: n(Resin-N+R 3 HCO 3 - ) +Xn-Æ(Resin-N+R 3 )nXn-+ nHCO 3 - where Xn-= F-, Cl-, NO 3 - , SO 42 - , PO 43 - etc. S ...

separation of enantiomeric forms of drugs having different pharmacological activities, chiral columns are expensive and most hav ...

172 Section D – Separation techniques 1 (^32) 6 4 5 Methanol (Meth) 7.5% Tetrahydrofuran (THF) 42.5% Water 50% Meth 4.5% THF 25. ...

Note how the elution order of the six components in the mixture alters with the mobile phase composition. Methods of identifying ...

Section D – Separation techniques D8 ELECTROPHORESIS AND ELECTROCHROMATOGRAPHY: PRINCIPLES AND INSTRUMENTATION Principles Electr ...

lamide or agarose, under the influence of an applied electric fieldthat creates a potential gradient. Two platinum electrodes (c ...

gels dissipate Joule heat more efficiently than column gels and have a superior resolving power. High-performance capillary elec ...

Capillary electrochromatography(CEC) is an even newer technique than CE and uses capillaries packed with 5mm or smaller particle ...

responsible for the movement of all species (cationic, anionic and neutral) along the capillary towards the detector and is the ...

surface to form an electrical double layer with an associated potential (zeta potential) (Fig. 3). Application of a voltage acro ...

Sample injection For classical electrophoresis, samples of 0.1–1 cm^3 are loaded into wells formed in gel slabs or layered onto ...

being the most common. DADs are increasingly preferred because of their ability to monitor at multiple selected wavelengths and ...

Section D – Separation techniques D9 ELECTROPHORESIS AND ELECTROCHROMATOGRAPHY: MODES, PROCEDURES AND APPLICATIONS There are sev ...

acids and peptides. A stable pH gradientis first created along the gel by polymerizing it with a mixture of polyamino-polycarbox ...

their distributions between the running buffer and the micelles. The general order of elution is: (i) cationic micelles and cati ...

D9 – Electrophoresis and electrochromatography: modes, procedures and applications 185 = Surfactant (negative charge) = Solute = ...

● capillary gel electrophoresis, CGE, is similar to classical gel electrophoresis, the capillary being filled with a polyacrylam ...

As yet, the number of applications is limited but is likely to grow as instru- mentation, mostly based on existing CE systems, a ...

Generally, only semi-quantitative information can be obtained from classical gel electrophoresis; visual comparisons can be made ...

Section E – Spectrometric techniques E1 ELECTROMAGNETIC RADIATION AND ENERGY LEVELS The nature of light and other radiation was ...