Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)

135 3(37,'( 3(37,'( 3(37,'( 3(37,'( 3(37,'( 65 5HDUUDQJHPHQW 65 3(37,'( 12,66(53;(71$1,%02&(5 &+(0,& ...

136 L. Leisle et al. generated by cleaving the intein fusion with a thiol like mercaptoethanesulfonic acid or ethanethiol. The p ...

137 The ligation chemistry requires a Cys residue and it is important to ensure that the Cys substitution does not adversely aff ...

138 L. Leisle et al. Further, isosteric manipulations that neutralize side-chains have been crucial in determining the roles of ...

139 Bertozzi ligation with triarylphosphine derivatives (Okuda and Tokuda 2009 ) or through 1,3-cycloaddition between the azido ...

140 L. Leisle et al. tions, as well as inter-subunit and -domain contacts (Schlieker 2004 ; Haslberger 2007 ; Boos et al. 2008 ; ...

141 Kir2.1, using a specifically evolved Ec Leu-RS variant paired with its cognate tR- NALeuCUA (Kang et al. 2013 ). The presenc ...

142 L. Leisle et al. to investigate the process of C-type inactivation. The following two modifications have been introduced usi ...

143 protein backbone of the selectivity filter. An ester linkage is iso-steric to an amide bond but shows reduced electronegativ ...

144 L. Leisle et al. References Adesnik H, Nicoll RA, England PM (2005) Photoinactivation of native AMPA receptors reveals their ...

145 Chen S, Schultz P, Brock A (2007) An improved system for the generation and analysis of mutant proteins containing unnatural ...

146 L. Leisle et al. Farrell IS, Toroney R, Hazen JL et al (2005) Photo-cross-linking interacting proteins with a geneti- cally ...

147 Kalstrup T, Blunck R (2013) Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid. ...

148 L. Leisle et al. Maquat LE (2004) Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics. Nat Rev Mol Cell Bi ...

149 Pless SA, Galpin JD, Frankel A, Ahern CA (2011a) Molecular basis for class Ib anti-arrhythmic inhibition of cardiac sodium c ...

150 L. Leisle et al. Santoro S, Wang L, Herberich B et al (2002) An efficient system for the evolution of aminoacyl- tRNA synthe ...

151 Wang K, Neumann H, Peak-Chew SY, Chin JW (2007a) Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic co ...

Index 153 A Acetylcholine receptor (AChR), 7, 8, 16, 21, 22, 57, 122 Acid–base chemistry, 4 Azobenzene, 79, 82, 85, 86, 89, 90, ...

154 Index limitations of, 68, 69 with functional, 56–58, 66 Substituted-cysteine accessibility method (SCAM), 6, 27–29, 38 Sulfh ...