Cannabis sativa L. - Botany and Biotechnology
THCA synthase catalyzes a unique monoterpene cyclase-like reaction coupled with a two-electron oxidation. However, the deduced p ...
and modulation were confirmed by site directed mutational analysis (Shoyama et al. 2012 ). For example, Tyr484 was found to most ...
this possibility, we studied the cell-specific expression and localization of THCA synthase in seed coat trichomes (Fig.8.6), an ...
8.2.2 Cannabidiolic Acid Synthase 8.2.2.1 Biochemical Properties CBDA is the dominant constituent of thefiber-typeC. sativa(also ...
transfer step: CBDA synthase abstracts a proton from the terminal methyl group of CBGA, whereas a proton is removed from the hyd ...
synthesized in young seedlings (Kushima et al. 1980 ). CBCA is derived from CBGA, and it was proposed that this biosynthetic rea ...
8.2.4 Geranyl Pyrophosphate:Olivetolate Geranyltransferase CBGA is an important cannabinoid as it is the central precursor of va ...
showed a high homology to various plastid localized aromatic prenyltransferases (Yazaki et al. 2009 ). Thus, it is likely that C ...
synthase.”The catalytic property of olivetol synthase was mysterious since olivetol is not a prenyl acceptor in the prenyltransf ...
barrel (DABB) protein that is structurally similar to polyketide cyclases found amongStreptomycesspecies. The recombinant OAC ca ...
8.3.1 Cannabinoid Production in C. sativa There are many ways to increase cannabinoid production inC. sativa. Varieties of this ...
toxic to the hairy root culture. EngineeringC. sativatissue cultures to overexpress genes involved in cannabinoid biosynthesis c ...
proteinase A. Overall, a much higher specific activity was obtained inP. pastoris. Whole cell bioconversion of CBGA in the recom ...
generated hairy roots expressing the mutant enzyme and after in vitro feeding of various strictosidine analogs, produced unnatur ...
Alaoui MA, Ibrahimi A, Semlali O, Tarhda Z, Marouane M, Najwa A, Soulaymani A, Fahime EE (2014) Affinity comparison of different ...
ElSohly MA, Slade D (2005) Chemical constituents of marijuana: the complex mixture of natural cannabinoids. Life Sci 78:539– 548 ...
Lindberg P, Park S, Melis A (2010) Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synecho ...
Saito K, Sudo H, Yamazaki M, Koseki-Nakamura M, Kitajima M, Takayama H, Aimi N (2001) Feasible production of camptothecin by hai ...
Taura F, Tanaka S, Taguchi C, Fukamizu T, Tanaka H, Shoyama Y, Morimoto S (2009b) Characterization of olivetol synthase, a polyk ...
Chapter 9 The Pharmacology and Therapeutic Potential of Plant Cannabinoids Maria Grazia Cascio, Roger G. Pertwee and Pietro Mari ...
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