AMPK Methods and Protocols
96-well plates for expansion. As colony formation could vary among cell types, optimal density of cell population needs to be de ...
protein modification across the clones using immunoblotting. Alternatively, we may imagine to directly assess protein expres- si ...
protein kinase signalings. PLoS One 11(8): e0161017 Wu YH, Li Q, Li P, Liu B (2016) GSK621 activates AMPK signaling to inhibit ...
Chapter 12 Compound C/Dorsomorphin: Its Use and Misuse as an AMPK Inhibitor Biplab Dasgupta and William Seibel Abstract The evol ...
including AMPK activators in basal and stress metabolism and in metabolic disease [12–14]. Conflicting reports about its cellula ...
type 1 receptor called ALK2 (ACVR1) [19–21]. In addition to inhibiting BMP signaling, dorsomorphin also inhibits the VEGF type 2 ...
molecule towards AMPK. For example, in a study involving a panel of 70 human kinases, Compound C was found to inhibit several ot ...
Compound C was even used to rescue the effects of AMPK activa- tors, although the effects of Compound C alone were not categori- ...
insufficient to rescue cells from the effect of Compound C. Instead, inhibition of the calpain-/cathepsin-mediated cell death pa ...
Viollet B, Andreelli F, Jorgensen SB, Perrin C, Geloen A, Flamez D, Mu J, Lenzner C, Baud O, Bennoun M, Gomas E, Nicolas G, Woj ...
identifies selective VEGF and BMP inhibitors. ACS Chem Biol 5(2):245–253.https://doi. org/10.1021/cb9002865 Bain J, Plater L, E ...
Chapter 13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation Jesper B. Birk ...
cells, fibroblasts, and different types of blood cells. Although in minority, these other cell types could contribute with AMPK ...
limit of Western blotting. If nearly all of a subunit isoform is coprecipitated, then the little remaining protein in the Post-I ...
2 Materials Prepare all solutions using ultrapure water (Milli-Q purified demi- neralized water) and analytical grade reagents. ...
Thus, to ensure a clean muscle sample, it is recommended that biopsy material is freeze-dried and subsequently dissected free of ...
Add IP buffer according to your calculations. If the total vol- ume of the IP should be 200μl, then the IP buffer should be 200 ...
Compare the signal from the different IPs to see how much of the different subunit isoforms are coprecipitated with the others. ...
Compare the Post-IPs to the Pre-IP to see how much of each specific subunit isoform is missing in the Post-IPs after the variou ...
buffer for each sample. This minimizes the time the muscle is at 4 C where enzymatic processes slowly start up. Once the tissue ...
«
6
7
8
9
10
11
12
13
14
15
»
Free download pdf