AMPK Methods and Protocols

AMPK Dietbert Neumann Benoit Viollet Editors Methods and Protocols Methods in Molecular Biology 1732 ...

METHODS INMOLECULARBIOLOGY Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield ...

AMPK Methods and Protocols Edited by Dietbert Neumann Department of Pathology, CARIM School for Cardiovascular Diseases, Maastri ...

Editors Dietbert Neumann Department of Pathology CARIM School for Cardiovascular Diseases Maastricht University Maastricht, The ...

Preface Since the discovery of AMPK in the early 1970s, this field garnered major interests among the scientists worldwide. Nowa ...

Contents Preface. .................................................................... v Contributors........................... ...

13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation. ..................... ...

27 Analyzing AMPK Function in the Hypothalamus........................... 433 Patricia Seoane-Collazo and Miguel Lopez 28 Using ...

Contributors FABIENNEANDRIS  Laboratoire d’Immunobiologie, Universite ́ Libre de Bruxelles—IBMM, Gosselies, Belgium JEAN-LUCBAL ...

MARCFORETZ  U1016, Institut Cochin, Inserm, Paris, France; UMR8104, CNRS, Paris, France; Universite ́ Paris Descartes, Paris, F ...

ARPITJAIN  Applied Bioinformatics Group, Institute of Cell Biology and Neuroscience, Goethe University Frankfurt, Frankfurt, Ge ...

Compostela, Spain; CIBER Fisiopatologı ́a de la Obesidad y Nutricion (CIBERobn), Santiago de Compostela, Spain BERNHARDLU ̈SCHE ...

NURIAM. PASTOR-SOLER  Division of Nephrology and Hypertension, Department of Medicine, USC/UKRO Kidney Research Center, Keck Sc ...

KATRINSPENGLER  Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Jena University Hospital, Jena, Germany ...

CHEN-SONGZHANG  State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life S ...

Chapter 1 Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (α 1 β 1 γ1) Julia A. Hubbard, Bing X ...

drug and metabolite (ADaM)-binding pocket. Ligand binding in the ADaM pocket occurs at the interface of the kinase and glycogen- ...

Variable-temperature shaking incubator with fittings for 12 2 L conical flasks. Centrifuges and rotors, e.g., Beckman-Coulter ...

2.3 Specialist Reagents and Equipment The protein purification protocol used here makes use of the A ̈KTA purification system ( ...

into several distinct stages, each broadly consisting of 3–4 days of laboratory work. In terms of hours worked in the laboratory ...