Synthetic Biology Parts, Devices and Applications

Index 407

societal actors 390
stakeholders 391
trans‐resveratrol 245–246
riboregulators 203
ribosomal binding sites (RBS) 331
riboswitch(es) 147, 185, 193, 198, 203
alternative splicing regulation in
eukaryotes 148
aptamer domain 147
expression platform 147
group I intron splicing regulation in
bacteria 148
ribozyme 183, 199, 204
RNA degradation process in E.coli 190
RNA interference
switches 170
utility 169
RNAi switches
applications 175, 177
design strategies 171
development 170
future aspects 177
oligonucleotide‐triggered 173
protein‐responsive shRNA
switch 176
protein‐triggered 174
rational design 174
RNAi pathway 170
small molecule‐triggered 171
RNA scaffolding 242
RNA switches
aptamers 181
complex riboswitches 185
expression platform 182–184
genetic selection 182
rational design 183
screening 182
transcriptional regulation 184
translational regulation 183
RNA synthetic biology 207
Rock’s Law 5
RRI see responsible research and
innovation (RRI)

s
science education 392
sDNA (synthetic DNA)

cost of 9

production methods 11

self‐splicing introns 136

shared DNA 19

small molecule‐triggered RNAi

switches 171

spliceosome 132

sRNA 195, 197–198

streamlined chassis 50

streamlined genome E. coli.

codon re‐assignment 70

gene function and network

regulation 69

genome architecture 70

mobile genetic elements, mutations

and evolution 69

testing hypotheses 68

streamlining

chassis 50

E. coli genome 51

genome reduction 64

random versus targeted 54

selection of deletion targets 55

substrate channeling 252

suicide genes 360–361

Superfunctionalized proteins 227

Surveyor nuclease 40

SynBio see synthetic biology (SynBio)

synthetic biology (SynBio) 81, 261

applications in health and

medicine 363

CARs 355

cellular therapies 350–351

chassis 96

chemically inducible caspase‐9 361

comparator 385–386

debates 388

engineering definition 81

genetic engineering 386

genome transplantation 83, 85

green biotechnology 385

helper functions 86

information technology 387

inverted cytokine receptors 358

master function 85

Maxwell’s demon 96

mechanosensitive channels 97