Wine Chemistry and Biochemistry

468 N. Terrier et al.

Ta b l e 9 B. 1

(continued)

Source

Reference

Detection

(–)-Epicatechin 3-gallate-(4

-8)-(–)-

epicatechin (B2 3 gallate)

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

(–)-Epicatechin-(4

-8)-(–)-epicatechin

3-gallate (B2 3’ gallate)

Berries

Czochanska et al.

(1979a)

PLC, -MS, -

1 HNMR

(–)-Epicatechin-(4

-8)-(–)-epicatechin

3-gallate (B2 3’ gallate)

Stem

Boukharta et al.

(1988)

LC-,UV spectroscopy, -

1 H NMR after enzymatic hydrolysis, acid hydrolysis,

or with

-thiol

(+)-Catechin-(4

-8)-(–)-epicatechin

3-gallate (B4 3 gallate)

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

(–)-Epicatechin 3-gallate-(4

-8)-(–)-

epicatechin 3-gallate (B2 3, 3’ digallate)

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

Flavanol trimers(–)-Epicatechin-(4

-8)- (–)-epicatechin-

(4

-8)-(–)- epicatechin (C1)

Wine

Lea et al. (1979)

PC, TLC after toluene-thiol hydrolysis

(–)-Epicatechin-(4

-8)-(–)-epicatechin-

(4

-8)-(+)-catechin

Wine

Lea et al. (1979)

PC, TLC after toluene-thiol hydrolysis

(–)-Epicatechin-(4

-8)-(–)-epicatechin-

(4

-6)-(+)-catechin

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, –

1 H NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

(–)-Epicatechin-(4

-6)-(–)-epicatechin-

(4

-8)-(–)-epicatechin

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

(–)-Epicatechin-(4

-8)-(–)-epicatechin-

(4

-6)-(–)-epicatechin

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol

(–)-Epicatechin-(4

-6)-(–)-epicatechin-

(4

-8)-(+)-catechin

Seeds

Ricardo da Silva

et al. (1991c)

HPLC, TLC, NMR, FAB-MS after enzymatic hydrolysis, acid hydrolysis,

partial acid-catalysed degradation with phloroglucinol orphenylmethanethiol