Quorum Sensing

Xylella fastidiosa............................................... 97, 243
Gram-positive bacteria
Bacillus subtilis...............................................118, 165
Geobacillus kaustophilus...........................................323
Staphylococcus aureus................................... 19,89–95,
363–365, 369
Staphylococcus epidermidis..............................364, 369
Streptomyces coelicolor....................................117, 118,
120–122, 124–126, 131–142
Streptomyces griseus..................................................118
Growth media
ATGN medium......................................................... 13
autoinducer bioassay (AB) medium........75,356, 358
boron-depleted media.........................................78, 82
brain heart infusion broth (BHI).......................91–93
Luria-Marine (LM) medium.............................75, 82,
356, 358, 359
lysogeny broth/Luria-Bertani broth (LB)13, 14, 289
M9 minimal medium................................................ 43
nematode growth medium (NGM)..... 329, 336, 337
supplemented minimal medium....................119, 120
terrific broth (TB) medium..................328, 329, 349
tryptic soy broth (TSB)..............................50, 52, 56,
57, 364, 365

H

Halogenated furanone..................................................276
Helper phage..............................329, 340, 343, 344, 346
High performance liquid chromatography (HPLC) ...27,
38, 40–43, 49, 50, 52, 54, 56, 57, 61–67,
69–71, 98, 99, 101, 120, 121, 164–166,
171–173, 175, 181, 195, 199, 215, 308,
364–368, 370
High-throughput screening................... 8,289, 298–300
Host–pathogen interaction..........................................204
Hybrid magnetic microparticles (HMP)..........64, 66, 70

I

Immunoblotting.........................................215, 220, 221
Immunofluorescence imaging......................................215
Immunoprecipitation...........................................219, 220
In-gel tryptic digestion.................................................195
Inhibition assay.............................................................8, 9
In planta movement......................................................251
In silico analysis............................................149–151, 190
In vitro
synthesis...............................................................90–91
imaging....................................................................282
In vivo infection models.....................228–230, 236–240
mouse models
abdominal burn infection model...................... 228
acute lung infection.......................................... 229
back burn infection..........................230, 236, 239

invasive infection of large-area burn

wounds......................................................... 229

open wound infection model ..........................230,

237–238, 240

lung infection...........................................203, 227

soft-tissue invasive wound infection................. 228

nematode killing assay ............................................336

IQ-motif-containing GTPase-activating protein

(IQGAP1)...........................................214, 223

Isobaric tags for relative and absolute quantitation

(iTRAQ) analysis.........................................194

Isovaleryl homoserine lactone (IV-HSL).........36, 40, 45

K

Kanamycin bioassay....................118, 119, 121, 124–127

Kinetic studies...............................................................173

L

Lectins.............................................................................. 26

Liquid chromatography/mass spectrometry

(LC/MS).................................. 27,49–58, 64,

91, 97–102, 104, 105

Liquid-liquid extraction (LLE) ..................108, 110, 111

LIVE/DEAD viability staining....................................282

Luciferase.......................................................................154

LuxR orphans or solos

classification...........................................147, 150, 151

identification...................................................148–151

ligands.............................................................146–153

M

Mammalian epithelial cells............................................224

Mass spectrometry (MS).....................................5, 36, 38,

49, 61, 71, 102, 104, 118, 199, 200

Mathematical models.................................253, 254, 256,

259, 262, 263, 268, 269

Messenger RNAs (mRNAs)................................178, 354

Metalloprotease............................................................... 74

Methylthioadenosine nucleosidase (MTAN)..............165,

170, 171

Microscopy

confocal scanning laser microscopy

(CSLM).....................206, 208, 210, 278, 282

epifluorescence microscopy....................................206

stimulated emission depletion (STED) super-

resolution microscopy........................222, 223

Minimum inhibitory concentration (MIC)................236,

365, 366

Monoclonal antibodies (mAbs).....................................38,

62, 325–352

Multiple reactions monitoring (MRM) scanning

mode.................................................50, 52, 54

Mutagenesis.................................................250, 323, 330

QUORUMSENSING

Index^373