Science - USA (2019-08-30)

reported same-sex sexual behavior across time, raising questions about how genetic and socio- cultural influences on sexual beha ...

Biol. 11 , e1004219 (2015). doi:10.1371/journal.pcbi.1004219; pmid: 25885710 S. R. Jaegeret al., A Mendelian trait for olfactor ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY Identification of a T follicular helper cell subset that drives anaphylactic IgE Uthaman G ...

RESEARCH ARTICLE ◥ IMMUNOLOGY Identification of a T follicular helper cell subset that drives anaphylactic IgE Uthaman Gowthaman ...

Gowthamanet al.,Science 365 , eaaw6433 (2019) 30 August 2019 2of14 A B C D E F G H I J Fig. 1. DOCK8 deficiency reveals the pres ...

Gowthamanet al.,Science 365 , eaaw6433 (2019) 30 August 2019 3of14 Fig. 2. TFH13 cells are induced in WT mice during aller- gic ...

high levels of serum IgE as they aged (fig. S2, F and G). Thus, T-Dock8−/−mice appear to reca- pitulate the hyper-IgE and dysgam ...

that the hyper-IgE phenotype in T-Dock8−/− mice is dependent on TFHcells but not on other cell types (e.g., CD4+DCs or CD8+Tcell ...

identified within the cTFHcell compartment of PN-allergic patients but not those of healthy controls (Fig. 4F). We also examined ...

that IL-4 production by TFHcells may be nec- essary for any IgE induction but insufficient for the production of high-affinity I ...

TH2 cells to Alt+OVA (Fig. 6A and fig. S15A). 13CreBcl6fl/flmice demonstrated minimal reduc- tion of total IgE and high-affinity ...

Cd4CreBcl6fl/flandIl13−/−mice as donors. TFH cells in these chimeras did not produce IL-13 but still made IL-4 to Alt+OVA immuni ...

T effector and TFHcells during multiple types of immune responses, but there is little reason to believe that a shared developme ...

manufacturer’s instructions or by fixing the cells with fixation buffer (BioLegend) for 20 min fol- lowed by permeabilization wi ...

experiment and version 3 chemistry for the second, and according to the manufacturer’s protocol (#CG00052) with modifications fo ...

In vivo T cell proliferation and TFHdifferentiation To assess proliferation, CD4+T cells from OT-II Dock8−/−(CD45.2+)orOT-IIWT(C ...

T. J. Looneyet al., Human B-cell isotype switching origins of IgE.J. Allergy Clin. Immunol. 137 , 579–586.e7 (2016). doi:10.101 ...

RESEARCH ARTICLE SUMMARY ◥ MALARIA Validation of the protein kinase PfCLK3 as a multistage cross-species malarial drug target Ma ...

RESEARCH ARTICLE ◥ MALARIA Validation of the protein kinase PfCLK3 as a multistage cross-species malarial drug target Mahmood M. ...

by a factor of ~100 (table S1). Similarly, TCMDC- 135051 showed no evidence of interacting with the human ortholog ofPfCLK3, PRP ...