Science 28Feb2020
RESEARCH ARTICLE SUMMARY ◥ CLINICAL TRIALS CRISPR-engineered T cells in patients with refractory cancer Edward A. Stadtmauer†, J ...
RESEARCH ARTICLE ◥ CLINICAL TRIALS CRISPR-engineered T cells in patients with refractory cancer Edward A. Stadtmauer1,2†, Joseph ...
on T cells to increase the safety and efficacy profile of NY-ESO-1 TCR–expressing engineered cells. In principle, this strategy ...
flow cytometric staining for Vb8.1ordextramer staining, ranging from 2 to 7% of T cells in the final product (Fig. 1C). The freq ...
Stadtmaueret al.,Science 367 , eaba7365 (2020) 28 February 2020 4of12 + Nalm-6 NY-ESO-1 Fig. 2. Potency and immunogenicity of CR ...
were infused with 1 × 10^8 cells/kg, and, owing to the considerable variation in TCR trans- duction efficiencies (table S2), the ...
increase the background in assays of off- target cleavage. The distribution of on- and off-target cleavage is expected to vary f ...
were identified that contained mutations in all three target sequences (Fig. 6, A and B). The most commonly mutated gene wasTRAC ...
( 19 , 21 – 24 ). We found that ~25% of the T cells expressing the NY-ESO-1 TCR in the infusion product had mutations in thePDCD ...
Stadtmaueret al.,Science 367 , eaba7365 (2020) 28 February 2020 9of12 IP 10 113 Time (Days) 0.0 0.2 0.4 0.6 0.8 1.0 Cell Proport ...
in the target antigens NY-ESO-1 and/or LAGE-1 (fig. S9). The reduction of target antigen was transient in patient UPN07 and pers ...
Discussion Our phase 1 first-in-human pilot study dem- onstrates the initial safety and feasibility of multiplex CRISPR-Cas9 T c ...
A. P. Rapoportet al., NY-ESO-1-specific TCR-engineered T cells mediate sustained antigen-specific antitumor effects in myeloma. ...
RESEARCH ARTICLE SUMMARY ◥ MICROBIOTA Maternal gut microbiota in pregnancy influences offspring metabolic phenotype in mice Ikuo ...
RESEARCH ARTICLE ◥ MICROBIOTA Maternal gut microbiota in pregnancy influences offspring metabolic phenotype in mice Ikuo Kimura1 ...
microbial community in infants ( 19 )andthus potentially influences the development of the host metabolic system. However, 16Sri ...
S7,AtoH).Furthermore,bothmaleandfe- male GF offspring from C57BL/6J mothers developed severe obesity and metabolic dis- orders u ...
Notably,Gpr43mRNA expression in both the colon and pancreas of GF embryos was sig- nificantly lower than that in SPF embryos, al ...
Kimuraet al.,Science 367 , eaaw8429 (2020) 28 February 2020 5of12 0 4 10 Relative mRNA expression (Ins2 /18S ) 8 6 2 * ** ** Pro ...
Kimuraet al.,Science 367 , eaaw8429 (2020) 28 February 2020 6of12 Fig. 5. Dietary fiber supplemen- tation of pregnant mothers re ...
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